| Employment Type: Permanent Staff (SHRA) Vacancy ID: P008423 Salary Range: $32,000 - $42,000 Position Summary/Description: The focus of the research is the study of HIV persistence (latency mechanisms, immune response, drug development, viral clearance, novel assays, approaches, and therapeutics). The research includes studies of signaling proteins and repressive transcription factors, studies of HIV infections in primary cells and cell lines and in humanized mice models with a strong focus on the in vitro and in vivo testing of small inhibitory molecules that target HIV latency through a variety of mechanisms of action, as well as evaluation of the immune function. The candidate will be expected to conduct experiments independently under the direction of laboratory PI and senior scientific leadership. The project goals and experimental plan will be defined by discussions with PI and the candidate will be expected to work with the PI and other laboratory researchers to design experiments and protocols to execute project goals. Experiments will include the use of following techniques and skill sets: cell culture under aseptic conditions, development of new cell based assays, RNA and DNA extraction, qPCR, protein isolation, gel electrophoresis, western blotting, immunoprecipitation, northern blotting, ELISAs, plasmid preparation and flow cytometry. The candidate will also provide support to faculty members, research scientists, post-doctoral trainees and graduate students in the lab by assisting with common laboratory duties and assays when necessary. Candidate will collaborate with PI and collaborators in the publication and presentation of research findings. Education and Experience: Demonstrated possession of the competencies necessary to perform the work. Essential Skills: Candidates must possess immunology and molecular biology knowledge, interest and skills; excellent aseptic tissue culturing skills for both cell lines and primary cells; flow cytometry experience; familiarity with quantitative/Real Time PCR and with PCR assay set up and analysis; and general experience with standard molecular biology assays. In addition to knowledge of basic molecular biology techniques and data analysis skills; must have strong interpersonal, verbal, and written communication skills; and must have the ability to work independently with guidance from the PI and senior scientific leadership. Preferred : Bachelor of Science in Biology, Microbiology, or a related field. At least one year of full-time lab experience in microbiology, immunology or molecular biology experience is preferred. Experience working with HIV and other sexually transmitted pathogens is preferred. Significant experience in working under BSL2 conditions; skilled in flow cytometry for both preparation of samples and analysis; skilled in cell based assay development; familiarity with western blotting; performing ELISAs, data acquisition and analysis is preferred. Experience with mouse handling, including husbandry, IP/IV injections, xenograft implantation and harvesting of tissues and blood is strongly preferred. AA/EEO Statement: The University of North Carolina at Chapel Hill is an equal opportunity and affirmative action employer. All qualified applicants will receive consideration for employment without regard to age, color, disability, gender, gender expression, gender identity, genetic information, race, national origin, religion, sex, sexual orientation, or status as a protected veteran. |
