The Research Assistant provides technical support to the Pathology faculty members in relation to their research projects. This position reports to the Assistant Professor, Pathology department. Work is performed under the direct supervision of one or two Pathology department faculty members to accomplish assigned projects. As a Research Assistant it will be necessary to follow established experimental protocols; assist in the development of new experimental protocols; assist with experiments; organize the laboratory; assimilate and evaluate data accurately; track ordering of equipment and supplies; and assist in research related activities. Good laboratory organizational skills and ability to work in a team environment are critical.
- Performs wide variety of standard research procedures with minimum supervision. Provides technical support for procedures used in all projects. Carries out experiments and assays according to experimental design, protocols, and procedures as coordinated with and approved by faculty member
- Mentor students working in the lab
- Trains students in laboratory safety and maintains required records
- Maintains and updates current knowledge base of related scientific literature, suggesting changes where needed to improve efficiency, sensitivity, and specificity of assays. Provides critical comments and suggestions on experimental procedures
- Confers with supervisor on a regular basis for analysis of data, assessment of progress and direction of research
- Records and maintains data
- Keeps an accurate lab notebook
- Contributes to abstract submissions and poster preparation
- Maintains paper work associated with lab safety protocols and attends all the required biosafety training
- Contributes to publication of research results
- Obtains quotes, performs ordering for lab supplies and reagents and keeps the laboratory appropriately stocked at all times
- Is responsible for all standard maintenance of the lab
- Aids supervisor in research related activities to include scheduling of experiments, organizing data for presentation and publication, and traveling to sites of collaboration in research projects
- Performs a variety of assays and experimental procedures including but not limited to:
- Propagation of mammalian cell lines using sterile/aseptic technique
- Conducts immunological assays including, but not limited to ELISA, flow cytometry, immunoblotting
- Conducts histology related work including fixation/processing of cells and tissues and special staining for cytological/histological analysis, immunohistochemistry, image analysis and digital acquisition
- Assists with animal studies including husbandry, in vivo xenograft models/experimental therapy studies, dissections/necropsy, blood collection, database management of colony
- Conducts molecular biology assays, such as RT-PCR, real-time PCR, and Western blot, plasmid preparation, cloning, mapping and sequencing of DNA, and other necessary steps for transfection experiments
- Prepares media, buffers, immunological, and biological reagents as needed
- Safely handles, records, tracks, and disposes of all hazardous and biohazardous materials, according to institutional guidelines
- All of above items will require that the Research Assistant will be proficient at many basic laboratory skills to include (but not limited to) sterile technique, calculating molarity, normality, accurate measuring, weighing, accurate pipetting, calculating dilutions and dilution factors, using a computer for data collection, and use of a microscope
- Performs additional duties as required
- Reports to Assistant Professor, Pathology.
- Has continuous contact with the department Administrative Assistant on matters of clerical, and administrative services
- Maintains contact with technical personnel within the Department of Pathology and other departments for technical assistance, use of other equipment and facilities, or for loan of reagents and supplies when deemed necessary
- Works well with and mentors students on their research projects as required
- May have limited contact with service representatives concerning equipment maintenance and with sales representatives concerning supplies and equipment
To perform this job successfully, an individual must be able to perform each essential duty satisfactorily. There are duties that require a significant degree of fine motor coordination. The requirements listed below are representative of the knowledge, skill, and/or ability required. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.
Education and Experience Requirement:
- Graduation from a recognized/accredited college or university with a Bachelor's degree in Molecular Biology or related biological sciences required. A minimum of 0-2 years of experience in Molecular Biology or related biological sciences is required.
- Persons with experience in molecular and cancer biology will be given interview preference. Experience in immunology is desirable.
- Must be willing to occasionally work late hours and weekends as needed
- Must be willing to work with rodents and infectious agents
Computer proficiency in MS Office (Word, Excel, Outlook, and PowerPoint).
Intermediate skills. Ability to read and interpret documents such as safety rules, operating and maintenance instructions and procedure manuals. Ability to write routine reports and correspondence. Ability to speak effectively before groups of customers or employees of organization.
Intermediate skills: Ability to apply common sense understanding to carry out instructions furnished in written, oral or diagram form. Ability to solve practical problems involving several concrete variables in standardized situations.
Basic skills. Ability to add, subtract, multiply, and divide all units of measure using whole numbers, common fractions and decimals. Ability to use formulas to calculate mass or volume of reagents required for specific or standardized solutions. Ability to compute rate, ratio, and percent and to interpret bar graphs is required
The physical demands described here are representative of those that must be met by an employee to successfully perform the essential functions of this job. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.
While performing the duties of this job, the employee is occasionally required to sit. The employee is regularly required to walk, climb or balance, stoop, kneel, crouch or crawl. The employee is frequently required to stand, use hands to handle or feel, reach with vision abilities required by this job include vision correctable to 20/20 in both eyes. The employee will regularly be required to lift/or move up to 20 pounds.
The work environments described here are representative of those an employee encounters while performing the essential functions of this job. The employee will be occasionally exposed to toxic or caustic chemicals. The noise level in the work environment is usually moderate.
Midwestern University is an Equal Opportunity/Affirmative Action employer that does not discriminate against an employee or applicant based upon race, color, religion, gender, national origin, disability, or veterans status, in accord with 41 C.F.R. 60-1.4(a), 250.5(a), 300.5(a) and 741.5(a).