Will work in established laboratory investigating mechanisms of acute lung injury using molecular biological and biochemical techniques in both tissue culture and in vivo models. After entry-level training under the general supervision of a faculty investigator or laboratory supervisor, is responsible for independently performing routine tests in a research laboratory and contributing to general maintenance of the laboratory, equipment and supplies.
Essential Job Functions:
Experiments will involve propagation of tissue culture, kinase assays, plasmid constructions, gel electrophoresis, immunohistochemical staining of cells and tissue, and some small animal surgery. [Research involves animal tissue and blood (non-A IDS-related)]. Follows established procedures or protocols and utilizes aseptic technique to perform laboratory tests and/or experiments which may include: basic tissue culture, basic molecular biology, and specialized histology techniques. Operates basic laboratory equipment such as centrifuge, pH meter, analytical balance, incubator, spectrophotometer, light microscope, and/or utilizes laminar flow hood. Operates lab computers for data entry. Utilizes in vivo models in experiments. Following experimental protocols, monitors animals and maintains health records in accordance with USDA and AALAC guidelines. Sacrifices and dissects animal remains as required. Prepares tissue for study utilizing biochemical and histochemical techniques. Maintains accurate data records.
Maintains and makes minor adjustments to equipment, e.g. calibrating, alignment. Complies with biohazard/radiation safety standards through proper handling of potentially hazardous chemical and biological agents and/or radiation sources in the workplace. Completes annual university biohazard/universal precaution/radiation safety training, as appropriate. Uses sterile techniques to avoid contaminating lab experiments. Uses universal safety precautions to protect self and co-workers from biohazardous materials, including blood-borne pathogens. Prepares solutions, reagents, and stains following standard laboratory formulas and procedures. Monitors inventory levels, orders materials and supplies in accordance with established policies and procedures, counts orders upon receipt.
Bachelor's degree in biology, chemistry, or related field required.
Must have a working knowledge of one or more of the following disciplines achieved through undergraduate studies, and laboratory experience gained through class work: biology, biochemistry, chemistry, physics, microbiology and/or genetics. Additional relevant laboratory experience acquired through extracurricular activities or summer employment is very desirable.
Johns Hopkins University remains committed to its founding principle, that education for all students should be grounded in exploration and discovery. Hopkins students are challenged not just to learn but also to advance learning itself. Critical thinking, problem solving, creativity, and entrepreneurship are all encouraged and nourished in this unique educational environment. After more than 130... years, Johns Hopkins remains a world leader in both teaching and research. Faculty members and their research colleagues at the university's Applied Physics Laboratory have each year since 1979 won Johns Hopkins more federal research and development funding than any other university. The university has nine academic divisions and campuses throughout the Baltimore-Washington area. The Krieger School of Arts and Sciences, the Whiting School of Engineering, the School of Education and the Carey Business School are based at the Homewood campus in northern Baltimore. The schools of Medicine, Public Health, and Nursing share a campus in east Baltimore with The Johns Hopkins Hospital. The Peabody Institute, a leading professional school of music, is located on Mount Vernon Place in downtown Baltimore. The Paul H. Nitze School of Advanced International Studies is located in Washington's Dupont Circle area.