The purpose of this position is to carry out the daily tasks of a Core facility laboratory. This position will set up sequencing reactions, gene expression and genotyping assays, distribute oligonucleotides and other products to customers, interact with and assist customers using self-service qPCR equipment. This position will interact with students, technicians and primary investigators as needed, including troubleshooting customer samples and experiments. This position must be able to provide exceptional customer service and maintain meticulous work standards.
% Time: 80% technical, 20% administrative
Duties and Responsibilities:
With minimal supervision, follow protocols to carry out sequencing reactions, gene expression assays, genotyping assays, pyrosequencing, digital PCR and other experiments as necessary. Follow QA/QC protocols. Responsible for daily maintenance of the equipment and proper use. Monitor lab supplies and inform supervisor when supplies are low. Follow all laboratory safety protocols. Assist customers as needed. Be able to troubleshoot customer experiments and provide assistance as needed. Keep track of self-service genotyping use and forward records to supervisor for billing. Ensure that client requests are entered properly into database. Ensure that samples are properly labeled and match requests. Contact customers as needed. Receive oligonucleotide delivers and orders and update database as necessary. Receive, log and track orders for delivery to our off-site locations. Deliver oligonucleotides and other products to customers as needed. Answer phones when needed. Must be willing to cross-train in human blood processing, cell culture and single cell genomic methods.
Other laboratory functions as assigned by supervisor.
Essential Job Functions:
Setting up sequencing reactions and running DNA Sequencer.
Setting up gene expression/genotyping assays and running associated equipment.
Interact with customers, assisting with troubleshooting.
Monitor lab supplies.
Reports to: DAF laboratory manager
Supervises: Student help, as needed.
Laboratory experience. Ability to perform repetitive and routine tasks while maintaining attention to detail. Experience with databases helpful. Must be able to interact professionally with customers.
Some laboratory experience (can be in college)
Special Knowledge, Skills, and Abilities:
Experience with the following equipment helpful, but will train as necessary: Centrifuges, electrophoresis equipment, 3730XL DNA Analyzers, Real-Time PCR equipment (including the QuantStudio qPCR instruments), PCR thermocycler, robotic and manual pipettors, common lab equipment (hotplate/stirrers, balances, spectrophotometer, etc.), Macintosh and IBM computers; 95% time.
Johns Hopkins University remains committed to its founding principle, that education for all students should be grounded in exploration and discovery. Hopkins students are challenged not just to learn but also to advance learning itself. Critical thinking, problem solving, creativity, and entrepreneurship are all encouraged and nourished in this unique educational environment. After more than 130... years, Johns Hopkins remains a world leader in both teaching and research. Faculty members and their research colleagues at the university's Applied Physics Laboratory have each year since 1979 won Johns Hopkins more federal research and development funding than any other university. The university has nine academic divisions and campuses throughout the Baltimore-Washington area. The Krieger School of Arts and Sciences, the Whiting School of Engineering, the School of Education and the Carey Business School are based at the Homewood campus in northern Baltimore. The schools of Medicine, Public Health, and Nursing share a campus in east Baltimore with The Johns Hopkins Hospital. The Peabody Institute, a leading professional school of music, is located on Mount Vernon Place in downtown Baltimore. The Paul H. Nitze School of Advanced International Studies is located in Washington's Dupont Circle area.