Summary Description: The Center for Infection and Immunity (CII) - one of the world's most advanced academic centers for research in infectious disease surveillance, diagnosis and discovery seeking an experienced Staff Associate to apply expertise in the application of molecular and computational biological methods in metagenomic studies focused on microbiome, mycobiome, microbial surveillance and discovery in health and disease. The candidate will perform experiments including preparation of bacterial 16S rRNA gene and fungal Internal Trascribed Spacer (ITS) gene libraries. In coordination with the PI, the candidate is expected to design experiments, independently execute and analyze results of sequencing and other experiments and report findings to the PI. S/he will also design and execute experiments involving PCR, cDNA cloning, nucleic acid extraction from tissues, cultures and clinical samples, cell and tissue culture, preparation of templates for DNA sequencing, preparation of templates for next generation sequencing. The incumbent will design assays for Real Time PCR analysis of bacterial and fungal organisms and will independently culture bacterial and fungal samples. In addition to experimentation, s/he will apply computational biology methods to high throughput sequencing projects relating to metagenomic surveys of microbial composition and function. S/he will assist in analyzing results of high throughput sequencing experiments. S/he will prepare mouse tissues and gastrointestinal tumors for nucleic acid and protein extraction and immunohistochemistry/immunofluorescence. Candidate will also perform other general laboratory related duties as assigned.Duties and Responsibilities:-Prepare samples for microbiome analysis with bacterial 16S, fungal 18s and Internal Transcribed Spacer (ITS) region and prepare bacterial and fungal amplicons for high throughput sequencing analysis -Apply computational biology methods to high throughput sequencing projects relating to metagenomic surveys of microbial composition and function -Design assays for Real Time PCR analysis of bacterial and fungal organisms and culture of bacterial and fungal samples -Prepare mouse tissues for nucleic acid and protein extraction and immunohistochemistry/immunofluorescence -Lead in the training of laboratory staff in the extraction of nucleic acids from bacterial and fungal organisms as well as methods for evaluation of 16s, fungal 18s and ITS analysis-Set up and carry out experiments involving PCR, cDNA cloning, nucleic acid extraction from tissue cultures and clinical samples, cell and tissue culture, preparation of templates for DNA sequencing, preparation of templates for pyrosequencing-Perform other laboratory related duties as assigned
Minimum Qualifications: Requires a Bachelor's degree in relevant field (e.g. molecular biology, microbiology, computational biology) plus 4 years experience or equivalent combination of education, training and experience.
Preferred Qualifications: Background in computational biology and/or experience with animal models of cancer.
As a member of the National Collegiate Athletic Association (NCAA) and the Council of Ivy Group Presidents (Ivy League), it is imperative that members of the Columbia University community, in all matters related to the intercollegiate athletics program, exhibit the highest professional standards and ethical behavior with regard to adherence to NCAA, Conference, University, and Department of Intercollegiate Athletics and Physical Education rules and regulations.
Columbia University is an Equal Opportunity/Affirmative Action employer.
Columbia University is one of the world's most important centers of research and at the same time a distinctive and distinguished learning environment for undergraduates and graduate students in many scholarly and professional fields. The University recognizes the importance of its location in New York City and seeks to link its research and teaching to the vast resources of a great metropolis. It... seeks to attract a diverse and international faculty and student body, to support research and teaching on global issues, and to create academic relationships with many countries and regions. It expects all areas of the university to advance knowledge and learning at the highest level and to convey the products of its efforts to the world.