This is a Research Associate 1 position for a virology laboratory under the direction of Dr. Yvonne Bryson Professor in Pediatric Infectious Diseases at DGSOM at UCLA, and under the supervision of our SRA3 Ruth Cortova. The research involves studies of HIV and other perinatally important viruses (including CMV). The position is funded by NIH RO1 and U19 grants and clinical trials. The work focuses on HIV CURE and pathogenesis of HIV in children and adolescents and pregnant women. The position is new,?requires knowledge of basic lab immunological and virological techniques including PCR and maintenance of cell lines tissue culture and virus stocks, and the interest in learning new assays.?This also requires processing and entering clinical specimens into the large data base using LDMS system. We are looking for a research associate who is flexible, enjoys experiments, and? is happy to help develop new techniques and aid with analyzing data and? with manuscripts. We have an ongoing study of innate antimicrobial activity of amniotic fluid and vernix from normal and HIV infected and exposed infants.
Skill in work organization and data handling. Working knowledge of serologic titers. Skill in using aseptic techniques required for microbiology and lymphocyte culture. Working knowledge of solution separation. Working knowledge of laboratory safety procedures associated with processing human body fluids. Ability to write clearly and concisely laboratory data, notes and directions and to label stored specimens clearly and accurately. Ability to respond to necessary changes in schedules and workloads cooperatively. Ability to speak on one-to-one basis using appropriate vocabulary and grammar to: a)obtain and convey information; b) discuss laboratory procedures Ability to work as part of a team. Ability to recognize problem situations and take appropriate action, including notification of appropriate personnel, if necessary. Ability to perform effectively under conditions of fluctuating workload. Effectively operate the following equipment:
Effective listening skills to understand tasks and assignments. Skill in following, modifying and preparing laboratory protocols as appropriate. Skill in computing cell concentrations and other needed laboratory calculations. Skill in setting priorities which accurately reflect relative importance of tasks and responsibilities. Skills in effective interpersonal interactions with all research staff (technical and professional) Ability to work independently and solve problems without immediate supervision Skill in accurate pipetting techniques Experience in handling radio isotopes Ability to enter repository location data in computer. Ability to move and hook up heavy liquid nitrogen tanks to freezers safely Availability Mon-Fri from 9AM-5PM
Knowledge of maintenance of tissue culture cell lines and virus stocks knowledge of PCR techniques real time PCR ,ability to extra DNA and RNA, knowledge or willingness to learn sequencing techniques ability to learn methods for digital drop PCR knowledge of flow techniques for lymphocyte sub set analysis familiarity with western blot techniques and elisa
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