This position is responsible for leading an NIH-funded study focused on the microbial ecology of lower airway disease in the Hunter lab in the Department of Microbiology & Immunology. The person hired will use a cystic fibrosis mouse model to assess nutrient acquisition mechanisms of bacterial pathogens in vivo. The ideal candidate will have experience with animal models, pathology, bacterial and epithelial cell culture, and genetic manipulation / analysis of bacteria using a variety of molecular approaches.
1) Maintenance of mouse colonies - 30% – Setting up mating, implementing mouse breeding and husbandry schemes, weaning and genotyping pups, administering drug treatments, daily health checks, maintaining up-to-date records/inventory.
2) Laboratory experiments - 50% - Bacterial culture (including anaerobic growth) and genetic manipulation - Bacterial agar bead preparation - Intratracheal and intrapulmonary mouse challenge with bacterial pathogens - Tissue harvesting and histochemistry - Tissue culture
3) Lab administration and maintenance - 10% - Ordering laboratory supplies - Setting up and maintaining lab equipment - Other lab organizational tasks as necessary - Maintaining meticulous lab notebooks (hardcopy and/or electronic) - Participating in and presenting research at group lab meetings
4) Mentoring - 10% - Assisting and training both undergraduate and graduate students with laboratory experiments
BS degree in Microbiology, Immunology, Biochemistry, Biology or related field, plus an advanced degree (MS, DVM, or PhD) with at least one year of relevant research experience Hands-on experience with mouse models
Molecular biology and computational biology expertise Experience developing IACUC protocols Experience working with cell culture Peer-reviewed publications
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.