This is a laboratory-based research position focused on the analysis of the molecular mechanisms of virus replication and particle assembly. The assigned research will utilize the techniques of molecular biology, biochemistry, mammalian tissue culture, and flow cytometry and cryo-electron microscopy in order to investigate the mechanisms of viral replication and particle assembly. High-resolution cryo-electron microscopy analysis will be conducted and studies directed at molecular genetic analysis of structure-function relationships will be pursued.
Conduct assigned research experiments on the molecular mechanisms of virus replication and particle assembly, that entail the use of the techniques of molecular biology, biochemistry, and microscopy analysis – 50%
Conduct assigned research experiments on the molecular mechanisms of virus replication and particle assembly, utilizing the use of the techniques of mammalian tissue culture, flow cytometry and microscopy – 40%
Lab maintenance and organization, including maintenance of lab equipment, general lab management and organization including tracking of lab supplies and reagents, capacity to develop skills to allow for the training of new researchers in lab procedures – 10%
Required/Essential Qualifications: (Must be documented)
A Bachelors degree in the biological sciences or relevant science discipline, or a combination of education and experience to total 4 years
Must be able to maintain bacterial cells in culture and induce protein expression
Must be able to perform biochemistry-related procedures: molecular cloning, gel electrophoresis, protein expression, sonication, FPLC protein purification
Course work in biochemistry, molecular biology & genetics, microbiology, immunology, and virology
Experience working with mammalian cell culture, both cell lines and primary cells, including freezing and thawing cells, making assay plates, transfections with plasmid constructs
Experience preparing samples for cryo-electron microscopy
Experience collecting high-resolution images by cryo-electron microscopy
Data preparation, interpretation and lab maintenance. Includes analyzing data, maintenance of lab equipment, general lab management and organization including tracking of lab supplies and reagents, capacity to develop skills to allow for the training of new researchers in lab procedures
Internal Number: 335349
About University of Minnesota, Twin Cities
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.