A cover letter is required for consideration for this position and should be attached as the first page of your resume. The cover letter should address your specific interest in the position and outline skills and experience that directly relate to this position.
The Mary H. Weiser Food Allergy Center (MHWFAC) (https://medicine.umich.edu/dept/food-allergy-center) seeks candidates with a M. S. degree in molecular biology and immunology with 5+ years of hands-on laboratory experience in molecular biology, tissue culture, viral vector expression systems, Lentivirus/ Retrovirus preparations and mammalian cell transduction methods and conditions. The primarily role for this position is to establish and maintain accurate inventory of parent cell lines and lentivirus/retrovirus transduced hematopoietic and non-hematopoietic cell lines.
The position would initially be for a 2-year period.
Daily culturing and maintenance of a variety of parental, immortalized mammalian cell lines.
Practice sterile tissue culture technique to ensure that cells are free of mycoplasma and other forms of contamination
New cell line generation. This will include usage of viral vector expression systems, Lentivirus/ Retrovirus preparations, • performing transductions, screening for single cell clones • Expansion of identified clones for analysis and screening • Freezing clones for storage.
Maintain an accurate hard copy and electronic laboratory documentation in appropriate systems (e.g. notebooks)
Follow established protocols for all practices including the culturing of cell lines, cell transduction preparation, and documentation.
Continuously seek, champion, and evaluate techniques and technological opportunities that will improve quality, efficiency, or scalability of cell culture
Conduct periodic cleaning and decontamination of Tissue Culture lab and quarterly cleaning of biosafety cabinets
Freezing of cell lines for storage, maintaining a local frozen cell bank to ensure vials are available at all times for major parental strains.
Establish and maintain an accurate inventory of cell lines.
M. S. degree in molecular biology and Immunology
5+ years of directly related laboratory experience in molecular biology, tissue culture, viral vector expression systems, Lentivirus/ Retrovirus preparations and mammalian cell transduction methods and conditions.
Working knowledge of principles of molecular biology, microbiology and immunology
Knowledge of lentiviral vectors, generation of lentivirus, and transduction of mammalian cells.
Ability to work autonomously with minimal supervision or collaboratively as part of a team on larger projects
Ability to communicate clearly the results of his/her experiments to other team members
Hands-on Cell Culture experience
Exceptional work ethic in a highly competitive environment
Strong written and verbal communication skills
Strong organizational skills analytical and communication skills are required for the position.
Attention to detail
Ability to multi-task and prioritize work effectively
Hands-on skills and experience in biochemical and molecular techniques including isolation of primary cells, quantification of mRNA (qPCR). cloning, and protein expression, biochemical and cellular screening assays
Experience in generation of intestinal organoids
Evidence of qualification is provided by previous employment, references, performance evaluations and/or personal interview
Michigan Medicine conducts background screening and pre-employment drug testing on job candidates upon acceptance of a contingent job offer and may use a third party administrator to conduct background screenings. Background screenings are performed in compliance with the Fair Credit Report Act. Pre-employment drug testing applies to all selected candidates, including new or additional faculty and staff appointments, as well as transfers from other U-M campuses.
Michigan Medicine improves the health of patients, populations and communities through excellence in education, patient care, community service, research and technology development, and through leadership activities in Michigan, nationally and internationally. Our mission is guided by our Strategic Principles and has three critical components; patient care, education and research that together enhance our contribution to society.
Job openings are posted for a minimum of seven calendar days. The review and selection process may begin as early as the eighth day after posting. This opening may be removed from posting boards and filled anytime after the minimum posting period has ended.
The University of Michigan is an equal opportunity/affirmative action employer.
Internal Number: 183923
About University of Michigan - Ann Arbor
A great university is made so by its faculty and staff, and Michigan is recognized as one of the best universities to work for in the country. The Michigan culture is known for engaging faculty and staff in all facets of the university to create a workplace that is vibrant and stimulating.For two consecutive years, the Chronicle of Higher Education has placed U-M in its "Great Colleges to Work For" survey. In particular, the university earns high marks for strong relations between faculty and administrators, a collaborative system of governance, strong pay and benefits, and a healthy work/life balance.