Research Technologist needed to manage daily operations of research laboratory investigating the dynamics of microbial pathogen exposures, immune responses, and human health.
Position will support research projects to investigate environmental and occupational exposures to microbial pathogens and human health outcomes. The primary focus of the lab is S. aureus, hepatitis E virus and influenza virus-related research including investigation of exposure and transmission routes, classification and molecular characterization of pathogens, and epidemiologic dynamics of immune responses and health outcomes. Work requires basic use of scientific methods, procedures and techniques; though training will be provided, previous experience and a profound understanding of theory behind experiments to perform data analysis and troubleshoot situations that need special attention with a substantial degree of independence are highly preferred.
Specific Duties & Responsibilities
Perform bacterial culture of environmental, human and animal specimens
- Testing of bacterial isolates for antimicrobial susceptibility (disk diffusion)
- Perform nucleic acid extraction (manual and automated)
- Perform RT-qPCR and endpoint PCR including polyacrylamide and agarose gel electrophoresis
- Prepare and test specimens for antibodies against pathogens and for pathogens (ELISA; Luminex)
- Maintain detailed records of quality assurance and quality control for specimens and specimen analyses;
- Record and analyze results, organize data, perform basic computations, and develop reports and summaries of analyses;
- Operate basic and advanced laboratory equipment, including automated nucleic acid extraction system, RT-qPCR instrument, microplate washer and reader, incubator, spectrophotometer, laminar flow hood, thermal cycler and other basic lab equipment.
Other responsibilities include:
- Contribute to general maintenance of the laboratory, equipment and supplies;
- Maintain and make minor adjustments to equipment, e.g. calibrations;
- Monitor inventory levels, order materials and supplies in accordance with established policies and maintain records.
Skills required: bacterial culture, isolation of bacteria and viruses, nucleic acid extraction, real time PCR, ELISA, Luminex multiplex immunoassays, cloning.
Minimum Qualifications (Required)
Bachelor's degree in biology, chemistry or related field.
Some related post-undergraduate work experience preferred.
Bachelor's degree in biology, chemistry, molecular biology, virology, microbiology, immunology or related field.
Experience in culture-based and molecular microbiology and immunology.
Experience in library prep for next generation sequencing and sequence analysis is preferred.
Special Knowledge, Skills, and Abilities:
Bacterial culture, isolation of bacteria and viruses, nucleic acid extraction, real time PCR, ELISA, Luminex multiplex immunoassays, cloning.
Equipment to be used: Nucleic acid extraction & qPCR instrument, Luminex instrument, ELISA reader.
Classified Title: Research Technologist Working Title: Research Technologist Role/Level/Range: ACRO37.5/02/CC Starting Hourly Pay Rate Range: $14.14 - $19.44/ Commensurate with Experience Employee group: Full Time Schedule: Monday - Friday; 8:30a-5:00p / 37.5 hours per week Exempt Status: Non-Exempt Location: 05-MD:School of Public Health Department name: 10001082-Environmental Health and Engineering Personnel area: School of Public Health
The successful candidate(s) for this position will be subject to a pre-employment background check.
If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at firstname.lastname@example.org. For TTY users, call via Maryland Relay or dial 711.
The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.
During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.
The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.
Equal Opportunity Employer Note: Job Postings are updated daily and remain online until filled.
Johns Hopkins University remains committed to its founding principle, that education for all students should be grounded in exploration and discovery. Hopkins students are challenged not just to learn but also to advance learning itself. Critical thinking, problem solving, creativity, and entrepreneurship are all encouraged and nourished in this unique educational environment. After more than 130 years, Johns Hopkins remains a world leader in both teaching and research. Faculty members and their research colleagues at the university's Applied Physics Laboratory have each year since 1979 won Johns Hopkins more federal research and development funding than any other university. The university has nine academic divisions and campuses throughout the Baltimore-Washington area. The Krieger School of Arts and Sciences, the Whiting School of Engineering, the School of Education and the Carey Business School are based at the Homewood campus in northern Baltimore. The schools of Medicine, Public Health, and Nursing share a campus in east Baltimore with The Johns Hopkins Hospital. The Peabody Institute, a leading professional school of music, is located on Mount Vernon Place in downtown Bal...timore. The Paul H. Nitze School of Advanced International Studies is located in Washington's Dupont Circle area.