This laboratory is investigating HIV persistence and latency using in vitro cell culture, molecular biology approaches, and humanized mouse models. The SRA will perform the following experimental procedures: Process blood samples; perform tissue culture; perform immunomagnetic separation of different populations of cells; grow and purify viral vectors and infectious HIV; infect primary cells and cell lines with HIV; perform molecular cloning reactions; perform humanized mouse surgeries; infect humanized mice with HIV; perform bleeds and process humanized mouse tissues; extract RNA/DNA/protein from different sources; perform PCR and sequencing; stain and analyze cells using flow cytometry; and maintain laboratory notebook. The SRA will also assist in general laboratory maintenance, ordering of supplies, maintenance of lab equipment and related records. Some flexibility in work schedule is required because occasional weekend and evening work is necessary.
B.S. in microbiology, biochemistry or molecular biology and at least 2 years laboratory experience.
Willingness to work with HIV.
Trained in mouse work.
Skill in reading and following instructions in laboratory protocols and other scientific documents.
Ability and willingness to work long hours and/or weekends on an intermittent basis.
Ability to perform a given protocol with meticulous precision and in a reproducible way with minimal supervision.
Ability to maintain accurate and timely records of experiments and equipment.
Skill in basic computer data acquisition procedures.
Knowledge of tissue culture and sterile techniques.
Ability to perform mathematical calculations for dilutions, molarity and molecular weight.
Skilled in use of common laboratory equipment using BSL2+ practices.
Trained in mouse survival surgeries.
May require flexible work schedule.
Final candidate subject to background check. As a federal contractor, UC Irvine is required to use E-Verify to confirm the work status of individuals assigned to perform substantial work under certain federal contracts/subcontracts.
The University of California, Irvine is an Equal Opportunity/Affirmative Action Employer advancing inclusive excellence. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, protected veteran status, or other protected categories covered by the UC nondiscrimination policy.
UCI provides reasonable accommodations for applicants with disabilities upon request. For more information, please contact Human Resources at (949) 824-0500.
Since 1965, the University of California, Irvine has combined the strengths of a major research university with the bounty of an incomparable Southern California location. UCI's unyielding commitment to rigorous academics, cutting-edge research, and leadership and character development makes the campus a driving force for innovation and discovery that serves our local, national and global communities.