Duties/Responsibilities: Manage the lab. Scheduling lab work/events, organizing supply inventory and requesting new supplies, managing animal protocols, inventory supplies and reagents in the lab to ensure proper supply and equipment is available and working. Make sure that lab is meeting any and all standards for lab safety and cleanliness. Order supplies and animals for coworkers and self as needed. Preparing supplies for use (i.e. autoclaving, obtaining and mixing chemicals, etc.) (25%)
Manage animal protocols. Obtain animals from specific IACUC protocol for coworkers for in vitro use in lab. Breed mice for experimental use and keep extensive records on all animal work. Work with and follow all IACUC and animal facility rules and regulations for lab. (10%)
Experimental design. Design, plan and schedule experiments either previously described or entirely new experiments in grant and IACUC protocols. Ensure all supplies/reagents needed for any and all experiments are on hand. (10%)
Perform Experiments. Harvest skin/blood/organs/mammary glands from mice to be processed and embedded in O.C.T. compound or paraffin blocks for staining. Perform immunohistological staining tissues to determine expression and/or localization of proteins of interest. Perform in vivo animal studies including tumor induction and promotion, label retaining skin stem cell assay, and UV carcinogenesis. Extract RNA/DNA/protein from cell cultured cells/single cell suspensions. Perform RT-qPCR, gel electrophoresis, and Western Blot. (30%)
Analyze Results. Record data from performed experiments in spreadsheets and compile records. Generate tables and/or graphs for recorded data. (10%)
Report/Communicate Results. Report analyzed data and figures to supervisor and coworkers at lab meetings. (10%)
Mentor/Coach Lab Employees. Coach and train new lab members and/or junior researchers to ensure smooth onboarding and compliance with institute and laboratory safety policies. (5%)
Required Qualification: B.A./B.S. in biochemistry with experience in microbiology, molecular biology and chemistry and 1 year of experience or education and experience in a specific or related filed of science related to the job totaling at least five years. Must be able to accommodate a flexible work schedule, including some hours in the evenings or weekends, as needed. Ability to work independently and rapidly acquire new skills in conduct of molecular or cellular procedures. Ability to work as part of a research team. 1-2 years of hands-on laboratory research and small animal research experience.
Preferred Qualifications: Experiences with RNA extraction, RT, qPCR, animal work (mice), DNA extraction, cell culture, tissue harvesting and procurement, animal surgical procedures, and flow cytometry are preferred. Knowledge and experience with research abstract and manuscript writing are desired.
Internal Number: 337208
About University of Minnesota, Twin Cities
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.