The Dick lab, in the College of Arts & Sciences, focuses on protein biochemistry and cryo electron tomography. We currently study how retroviruses like HIV assemble into virions. The Dick lab an individual to carry out basic research related to cloning, protein expression and purification, and setting up and screening protein assembly reactions. We are looking for a enthusiastic individual who works well with others in a collaborative environment. In addition to basic molecular biology and biochemistry work, the individual will also be responsible for maintaining the lab environment. This may include but not be limited to, general cleanliness, preparation of media and buffers, cleaning glassware, procuring suplies, and maintaining lab equipment.
Responsibilities include but are not limited to:
Assist in conducting research experiments using molecular biological techniques such as PCR (including design of primers and trouble shooting), site-directed mutagenesis, DNA cloning and protein expression in E. coli. Have primary responsibility for protein purification from E. coli (including affinity, ion exchange, and size exclusion chromatography) and analysis. Screening of protein by temperature shift assays and transmision electron microscopy. Have primary responsibility for mammalian cell culture (cell passage, DNA transfection, freezing, and immune-fluorescence microscopy. Prepare and maintain stock solutions. Maintain lab notebook records. Order and maintain laboratory supplies. Adhere to lab safety protocols with a solid understanding of laboratory safety techniques and procedures. Ensure department compliance with regulations on storage, labeling, handling, and proper waste disposal. Maintain overall laboratory order and organization.
This is a 12-month term appointment with the possibility of extension contingent on successful performance and continued funding
Associate's degree or other formal training program of two years or equivalent; more than 1 year, but less than two years or equivalent combination of education and experience.
Must be personable, flexible, proactive, demonstrate a positive "can do" attitude, possess excellent organizational and communication skills and take pride in their work.Ability to develop effective working relationships with students, faculty and staff.
Ability to maintain a comprehensive, up to date and accurate research lab notebook.Visual concentration, attention to detail and manual dexterity are required.
Must be willing to learn new laboratory skills. Must be able to work independently as well as in a team environment to accomplish required tasks.
Should be comfortable with basic computer programs such as MS Word and Excel.
Able to work under general supervision and proceed alone on regular tasks.
Bachelor's degree in biology, molecular biology, biochemistry, or cell biology, or related subject.
Knowledge of protein biochemistry, structural biology, cryo-EM, or basic computational biology a plus.
Experience with eukaryotic cell expression and tissue culture highly desirable but not required.
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