Rutgers, The State University of New Jersey is seeking a motivated Research Associate II for the Department of Microbiology, Biochemistry and Molecular Genetics within the New Jersey Medical School.
A Research Associate position is available in the Petrou lab at Rutgers-New Jersey Medical School (Newark, NJ). Our research focuses on the structural biology of membrane proteins using single particle cryoEM. Our goal is to characterize the structure and function of bacterial membrane enzymes involved in antibiotic resistance, and eukaryotic receptors and enzymes relevant to human physiology and pathology. The Petrou Lab is located in Newark, New Jersey, 20 min away from New York city. The Rutgers New Jersey CryoEM/ET facility, located in Piscataway, New Jersey, provides access to cryoEM and cryoET instrumentation including a 200kV Talos Arctica microscope, an Aquilos 2 FIB-SEM microscope, Leica cryo-CLEM setup, and other pertinent equipment (i.e. Vitrobot, glow dischargers, etc.). A screening facility that will house a 120kV Talos L120C microscope equipped with speed-enhanced Ceta camera, and sample preparation equipment, is being commissioned and will be located in the same building as the Petrou Lab. The Research Associate will be responsible for the expression, purification and structure determination by single-particle cryoEM of membrane proteins relevant to polymyxin antibiotic resistance. Moreover, the Research Associate will assist the PI in lab organization, daily operations, personnel training, and grant writing activities. The candidate will have a Ph.D. in Structural Biology, Molecular Biology, Biochemistry, or related fields. At least three years of postdoctoral experience in membrane protein expression and purification and/or single-particle cryoEM, and evidence of academic productivity are required. Good communication & organizational skills and the ability to work independently are important for this position. Salary will be commensurate to the candidate's experience and qualifications. Interested candidates should e-mail a short cover letter detailing their background, CV and a list of three references to Dr. Vasileios Petrou (email@example.com).
Among the key duties of this position are the following:
Performs the following research-related tasks: a.Determination of three-dimensional structures of proteins using cryo-electron microscopy data and appropriate computational programs/resources (e.g. Relion, cryoSPARC, etc.). b.Sample preparation for single-particle cryoEM using cryo-plunging equipment (i.e. Vitrobot or equivalent). c.Screening and data collection of single-particle samples using a cryo-transfer holder and side-entry transmission electron microscope. d.Protein expression and purification – overexpression using bacterial systems, SDS-PAGE, and chromatographic separation using FPLC and HPLC. e.Biochemical characterization of recombinant and native proteins (Western blotting, immunoprecipitation, cross-linking, etc.) f.Molecular biology (expression vector design and construction, PCR, site-directed mutagenesis, in vitro transcription, etc.)
Collects, compiles and presents data with appropriate conclusions, recommendations, etc. pertinent to research activity.
Communicates regularly with the P.I.
Maintains detailed and accurate experimental records.
Participates and presents in journal clubs, seminars and conferences.
Prepares reports for peer-reviewed scientific publications, and contributes to grant writing.
Performs training of lab personnel in molecular biology, biochemistry and single-particle cryoEM.
Supports the daily operations within the lab, e.g., lab reagent generation and administrative tasks
Performs laboratory inventory monitoring and ordering.
Minimum Education and Experience:
Ph.D. in Structural Biology, Molecular Biology, Biochemistry, or related fields.
Minimum three years postdoctoral experience in Molecular Biology, Biochemistry, Cell Biology, or related fields.
Strong background in membrane protein expression and purification and/or single-particle cryoEM.
Good communication & organizational skills and the ability to work independently are important for this position.
The candidate should include a cover letter and contact information for three references with their application.
Physical Demands and Work Environment:
PHYSICALDEMANDS: Must be able to safely perform laboratory experiments.
WORKENVIRONMENT: Research laboratory
Posting Number: 20ST1750
Location: Newark (RBHS)
Internal Number: 120240
About Rutgers University
Rutgers, The State University of New Jersey, is a leading national public research university and the state's preeminent, comprehensive public institution of higher education. Rutgers is dedicated to teaching that meets the highest standards of excellence; to conducting research that breaks new ground; and to turning knowledge into solutions for local, national, and global communities. As it was at our founding in 1766, the heart of our mission is preparing students to become productive members of society and good citizens of the world. Rutgers teaches across the full educational spectrum: preschool to precollege; undergraduate to graduate and postdoctoral; and continuing education for professional and personal advancement. Rutgers is New Jersey's land-grant institution and one of the nation's foremost research universities, and as such, we educate, make discoveries, serve as an engine of economic growth, and generate ideas for improving people's lives.