The mission of the Indiana University School of Medicine, Department of Ophthalmology is to train tomorrow's ophthalmologists and advance treatments and prevention of eye diseases and vision disorders through basic, translational, and clinical research.
Our research program continues to grow as our scientists are being awarded impressive grants and are bringing the results of their research to the medical and scientific community.
We seek an individual with excellent communication skills, independence, and drive to join our research group under the direction of Dr. Tasneem Sharma. Dr. Sharma conducts glaucoma research with specialization in perfusion organ culture systems, neuron regeneration, therapeutics, and stem cell differentiation.
The Research Analyst I coordinates laboratory activities and monitors the progression of research projects. Supervises research technicians and student employees. Participates in protocol development, data analysis and report preparation. The position requires expertise in the application and adaptation of various complex research techniques and procedures.
Duties and responsibilities include:
Design, perform, and/or oversee experiments and data collection.
Conduct a wide range of complex experiments with a degree of independence in conducting glaucoma and stem cell research.
Perform complex techniques or procedures including but not limited to:
Basic molecular techniques like PCR, qPCR, western blots and IHC.
Identify and validate AAV2 therapies for neuroprotection & regeneration.
Analyze bioinformatic analysis of microarray gene expression datasets.
Application of gene therapy in patient-specific retinal cells.
Differentiate patient hiPSCs to retinal ganglion cells and generation of 3D retinal organoids.
Correct mutations in patient-specific hiPSCs with CRISPR-Cas9 based genome editing.
Perform RNA-Seq analysis on various neuronal subtypes subjected to different pathogenic conditions.
Utilize confocal and fluorescence microscopy to image 3D hydrogels and cells.
Utilize fundamental concepts, practices, and procedures to perform routine scientific research tasks.
Use in vitro cell culture and ex vivo perfusion organ culture systems and utilization of therapeutic interventions.
Ensure data integrity, quality control, and protocol compliance.
Coordinate the assignments of laboratory personnel; monitor completion of assigned tasks and provide training to research technicians and/or graduate students as needed.
Train laboratory personnel in various techniques and analysis.
Assist in preparing completed research reports or analyses on each project phase.
Direct or participate in the tabulation and computation of project results.
Write results of experiments and assist in writing articles for publication.
Participate in the development of grant protocols.
Serve as a resource to other laboratory personnel.
Supervise the set-up and maintenance of standard and/or specialized apparatus or laboratory equipment.
Maintain knowledge of relevant state-of-the-art technology, equipment, and/or systems.
Oversee disposal of radioactive waste.
Maintain safety records and obtain special permits as required.
Maintain knowledge of scientific trends through readings, conferences, and seminars.
Review literature to identify suitable procedures and methods.
Promote continuous improvement of workplace safety and environmental practices.
Bachelor's degree in science and three years of research experience.
Expertise in the application and adaptation of numerous research techniques and procedures. Knowledge of differentiating patient hiPSCs to retinal ganglion cells and generation of 3D retinal organoids. Knowledge and experience using in vitro cell culture and ex vivo perfusion organ culture systems and utilization of therapeutic interventions. Excellent communication skills.
Master's degree in science. Experience with molecular biology, stem cell biology, ex-vivo perfusion culture and/or mouse breeding as well as rodent ocular surgery. Ability to exercise independent judgment. Ability to interpret and determine appropriateness of scientific readings. Knowledge of laboratory techniques, and protocols. Knowledge of laboratory practices and ability to train new hires; notebook maintenance, and specific assay protocols. Successful completion of the required courses through the safety department (Radiation, Blood Bourne Pathogens, Biological and Laboratory Safety, etc.).
Working Conditions / Demands
Research Laboratory. Animal subjects. Some experiments may require long periods of time in a stationary position. Other lab duties may involve lifting heavy equipment or materials.
Must be able to perform the essential functions of the position with or without reasonable accommodation.
Salary Plan: PAE
Salary Grade: 3RS
Job Function: Research
Due to the COVID-19 pandemic, there may be differences in the working conditions as advertised in our standard job postings (e.g., the ability to travel from one campus to another, etc.). If you are invited for an interview, please discuss your questions or concerns regarding the working conditions at that time.
This posting is scheduled to close at 11:59 pm EST on the advertised Close Date. This posting may be closed at any time at the discretion of the University, but will remain open for a minimum of 5 business days. To guarantee full consideration, please submit your application within 5 business days of the Posted Date.
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Equal Employment Opportunity
Indiana University is an equal employment and affirmative action employer and a provider of ADA services. All qualified applicants will receive consideration for employment without regard to age, ethnicity, color, race, religion, sex, sexual orientation, gender identity or expression, genetic information, marital status, national origin, disability status or protected veteran status. Indiana University does not discriminate on the basis of sex in its educational programs and activities, including employment and admission, as required by Title IX. Questions or complaints regarding Title IX may be referred to the U.S. Department of Education Office for Civil Rights or the university Title IX Coordinator. See Indiana University's Notice of Non-Discrimination here which includes contact information .
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Founded in 1820, Indiana University is the state’s public university system, comprised of two core campuses—IU-Bloomington and IUPUI—and six regional campuses located throughout the state. Combined, the total student population is more than 114,000 undergraduate, graduate and professional school students. IU is one of the largest employers in the state, with over 20,000 faculty and staff. Indiana University is an equal employment and affirmative action employer and a provider of ADA services. Diversity and inclusion remains a core commitment to IU’s mission to cultivate an educational environment that supports equal access, participation and representation on all of its campuses and that provides educational and career opportunities for all.