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The UW Department of Immunology has an excellent opportunity for a Research Scientist 3 in the Oberst Lab.
Research in the Oberst lab focuses on understanding how different forms of cell death occur, and how they influence immune responses in vivo. Multicellular organisms are complex communities of cooperating cells, and the death of some cells is required for the community to survive and thrive. It has classically been thought that cells can die in one of two ways: (1) Necrosis, a passive form of cell death, which occurs in response to overwhelming damage, and (2) Apoptosis, which is a regulated form of cellular suicide that is carried out by specific proteins in response to discrete stimuli. Importantly, necrotic cell death involves cellular rupture and the release of potentially damaging cellular contents into surrounding tissue, leading to inflammation. Apoptosis, by contrast, is an ordered process of cellular disposal in which cells are disassembled, packaged, and rapidly eliminated by phagocytes; it is therefore non-immunogenic in most circumstances.
The responsibilities of the position will include carrying out tissue culture experiments, performing high-throughput sequencing and array experiments and creating new cell lines.
Specific responsibilities include: •Conducting literature reviews to support the lab's research. •Using accepted methods and techniques to solve well-defined problems. •Performing specific and limited portions of broader projects, under the technical guidance of an experienced senior research scientist. •Cell biology and cell culture: Fluorescence microscopy; flow cytometry; transfections; transformations; culture of bacterial and vertebrate cells. •Molecular genetics: Preparation and analysis of DNA from plasmids, cell culture and primary cells; cloning. Purification of recombinant proteins; protein analysis by gel electrophoresis and western blotting; enzyme activity assays. ChIP, ChiP-Seq, and genomic analyses including DNA sequencing and RNA-Seq. •Somatic cell genetics: Generate mutant and transgenic cell lines for analysis. •Computational analysis of experimental results. •Interacting collaboratively with the laboratory research team. •Contributing results, text, and images for grant submissions and presentations. •Presenting research results at laboratory, departmental, and national meetings. •Other duties as assigned.
MINIMUM REQUIREMENTS •Doctoral degree in immunology, cellular biology, or microbiology. •Three to five years of relevant laboratory research experience. •Broad knowledge of the principles, practices, and procedures involved with cell biology and cell culture, molecular genetics, and somatic cell genetics.
Equivalent education and/or experience may substitute for minimum requirements.
DESIRED REQUIREMENTS •Experience with computational analyses of cellular processes. •Experience presenting research findings. •Ability to mentor graduate students.
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