Baxter Laboratory is seeking a temporary life sciences technician. This position will support the development of a highly scalable multiplexed serological assay for COVID-19. We will expand this assay to detect antibodies against different pathogens simultaneously. An additional goal is to expand multiplex testing to viral genomes and antigens.
Will perform experiments related to mass cytometry, immunofluorescence and sequencing.
Will be involved with collecting, storing, and processing SARS-CoV-2 samples.
This position requires the ability to troubleshoot experimental assays in conjunction with other Nolan lab members.
Will maintain and manage assay stocks/reagents (e.g., preparation of buffers, blocking reagents, inventory of oligos, isotopes and antibodies)
Perform typical flow cytometry and microscopy techniques
Perform cell culture and bacterial growth for protein synthesis
Additionally, cell culture, cell count, RNA expression, protein quantification will periodically be required. Perform molecular biology protocols to verify conjugations (PCR, Bradford Stains, SDS-page gels)
Automation using robotics will consist a portion of the assay development
Aid in data analysis processing using established software, ImageJ, and some basic R code.
The position includes maintaining detail records of performed experiments and their outcome. This is achieved in the form of a lab notebook where experiments and their results are recorded daily.
EDUCATION AND EXPERIENCE:
Bachelor's degree in a STEM field.
Must have > 2 years' experience in a molecular biology lab.
Experience with automation principles (robotics, coding) is a plus.
DESIRED QUALIFICATIONS: Bachelor's degree in Biology, Biochemistry, Bioengineering, Biophysics, or college level coursework in a related discipline. Extensive prior experience with single-cell mass cytometry, fluorescence-based flow cytometry, sequencing, or characterization of cell lines. Excellent laboratory management skills and knowledge of the full range of genomic, proteomic, biochemical and immunohistochemical laboratory techniques. Familiarity with and adherence to laboratory safety best-practices and Stanford University Health and Safety policies and regulations. Working closely on a team as a member and as a leader on specific research projects is essential, sharing data, training colleagues and trainees in new techniques and protocols, documenting research protocols and results and recording and analyzing data is key. The candidate will also assist with preparation of data, figures and reporting for manuscripts, progress reports and grant submissions: Proficiency in English and strong writing skills are desired.
Stanford is an equal opportunity employer and all qualified applicants will receive consideration without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, veteran status, or any other characteristic protected by law.
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Located between San Francisco and San Jose in the heart of Silicon Valley, Stanford University is recognized as one of the world's leading research and teaching institutions. Leland and Jane Stanford founded the University to "promote the public welfare by exercising an influence on behalf of humanity and civilization." Stanford opened its doors in 1891, and more than a century later, it remains dedicated to finding solutions to the great challenges of the day and to preparing students for leadership in a complex world. The University's thriving diverse community is comprised of nearly 7000 undergraduate students, 12000 graduate students, 2000 faculty members, 1900 postdoctoral scholars, and over 11,000 academic and administrative staff in seven schools including several interdisciplinary research centers and institutes. The campus spreads over 8000 contiguous acres and nearly all undergraduates live on campus. Stanford offers bachelor's and master's degrees in addition to doctoral degrees (PhD, MD, DMA and JD) plus a number of professional and continuing education programs and certifications. More at http://facts.stanford.edu and http://www.stanford.edu. Stanford is an equal empl...oyment opportunity and affirmative action employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, protected veteran status, or any other characteristic protected by law. Stanford also welcomes applications from others who would bring additional dimensions to the University’s research, teaching and clinical missions.