The Department of Laboratory Medicine and Pathology serves as a regional resource for clinical laboratory services required for patient care, research and educational programs in laboratory medicine and pathology. The department now employs 900 people who work at the award-winning University of Washington Medical Center, Harborview Medical Center and many other clinical and research facilities in the area. The University of Washington (UW) is proud to be one of the nation’s premier educational and research institutions. Our people are the most important asset in our pursuit of achieving excellence in education, research, and community service. Our staff not only enjoys outstanding benefits and professional growth opportunities, but also an environment noted for diversity, community involvement, intellectual excitement and natural beauty.
Our LABORATORY MEDICINE AND PATHOLOGY DEPARTMENT has an outstanding full-time position for a day shift Research Scientist 3. The Research Scientist/Engineer 3 will provide support for NIH-funded projects examining the genetics, biochemistry, and cell biology of host-pathogen interactions in the laboratory of Dr. Ferric Fang. The focus of the research effort in the Fang lab is to understand basic mechanisms of bacterial pathogenesis using Salmonella Typhimurium, Salmonella Typhi and Salmonella Paratyphi A. The applicant will be required to conduct in vitro and in vivo experiments with the above organisms in a Biosafety Level 2 and Animal Biosafety Level 2 laboratory. The position has both managerial and experimental responsibilities including general laboratory management and mentoring of graduate and undergraduate students, and the applicant should be able to work independently.
Design and perform experiments using bacterial pathogens, mammalian cell culture and murine models of infection developing new protocols, and/or improving established methodologies
Responsible for overall operation of the laboratory and implementation of laboratory safety policies
Perform experiments using molecular, genetic and biochemical methods and optimizing methods as required.
Construction of libraries for next generation sequencing (NGS) and perform bioinformatic data analysis.
Utilize lambda-red mediated genetic engineering for generation of recombinant strains.
Plasmid construction using a variety of DNA cloning techniques including Gibson Assembly.
Perform and optimize PCR and qPCR assays, Western Blot hybridization, immunoprecipitation.
Perform experiments using cell culture, murine and humanized animal models.
Knowledgeable with regulatory compliances for UW institutional settings. Responsible for overall operation of the laboratory and implementation of policies.
Participate in collaborative research projects.
Prepare manuscripts for publication.
Contribute towards grant submissions.
REQUIREMENTS Masters degree in microbiology, chemistry, biochemistry, molecular or cellular biology or a related field AND three years of related, professional laboratory experience.
ADDITIONAL REQUIREMENTS Proficiency working with research mice and infection models.
An equivalent combination of education and experience may substitute for stated requirements.
Molecular microbiologist with experience in assay development and optimization for molecular and biochemical methods
Experience in DNA/RNA methodologies including library constructions for next generation sequencing (NGS) including data analysis
Organized with laboratory management skills
Highly motivated, enthusiastic, and willing to learn new techniques
Willing to participate in collaborative research projects within the laboratory
Having a demonstrated ability to work with mice and infection models
UW Medicine includes Harborview Medical Center, UW Medical Center - Montlake, UW Medical Center - Northwest, Valley Medical Center, UW Neighborhood Clinics, UW Physicians, UW School of Medicine, and Airlift Northwest.
Founded in 1861, the University of Washington is one of the oldest public institutions in the west coast and one of the preeminent research universities in the world. The University of Washington is a multi-campus university comprised of three different campuses: Seattle, Tacoma, and Bothell. The Seattle campus is made up of sixteen schools and colleges that serve students ranging from an undergraduate level to a doctoral level. The university is home to world-class libraries, arts, music, drama, and sports, as well as the highest quality medical care in Washington State and a world-class academic medical center. The teaching and research of the University’s many professional schools provide undergraduate and graduate students the education necessary toward achieving an excellence that will serve the state, the region, and the nation. As part of a large and diverse community, the University of Washington serves more students than any other institution in the Northwest.