Research Assistant I position will focus on animal research support, histology, microscopy, and molecular biology under the direct supervision of a Staff Scientist and close collaboration with lab Postdocs. Position will be trained in specific techniques and methods in order to execute experiments designed by Staff Scientist or Postdocs. Research Assistant will be expected to carry out, analyze, and present experimental results to research team under close supervision. Candidate must be willing to work in an animal research environment and perform transplantation surgeries involving human cells.
This position supports two projects in the lab: 1) understanding the development and function of human neurons and glia using transplantation into a host mouse brain and 2) understanding the contribution of hippocampal neurons to learning and memory.
(70%) Animal Research Support
Perform rodent drug dosing by multiple routes (e.g. injection, gavage).
Perform rodent surgeries and manage post-operative care of animals.
Perform mouse behavioral tests and analysis of behavioral data
Maintain transgenic mouse colonies, including breeding, tagging, and genotyping.
Collect, section, and perform immunofluorescence protocols on fixed animal tissues.
Analyze cells and tissues using fluorescent and confocal microscopes.
(20%) Molecular Biology
Perform various techniques in molecular biology such as:
RNA extraction and analysis.
DNA extraction and analysis.
PCR and rtPCR.
(10%) Lab Support
Prepare standard solutions.
Stock and organize incoming lab supplies.
Attend staff meetings and assist with general problem solving.
Assist in hazardous, biohazardous and waste disposal.
Assist with maintaining laboratory safety records.
No professional experience required with relevant degree
Demonstrated experience in rodent surgery, particularly intracranial surgeries.
Demonstrated experience in analysis of animal behavior, histology, and molecular biology data.
Demonstrated experience with confocal imaging and imaging analysis.
1-2 years of laboratory experience or equivalent combination of education and experience
1 year of rodent handling experience
Knowledge of histology, microscopy, and molecular biology techniques.
Proficient in the operation of standard lab equipment and computer hardware/software.
Bachelor's degree in biological sciences or equivalent
SKILLS AND ABILITIES
Theoretical and hands on knowledge of rodent handling, surgery, histology, microscopy, tissue culture, and molecular biology techniques.
Demonstrated knowledge and experience in researching information and analyzing data to arrive at valid conclusions.
Ability to manage and track large numbers of samples meticulously without mixup or cross contamination.
Skill in the operation and maintenance of various lab equipment.
Demonstrated knowledge and experience with both PC and MAC operating systems, standard business office equipment and commonly used software and tools, such as Microsoft Office, electronic mail, and the internet.
Proficiency with common office software applications as well as software applications used for research, recording, and analysis.
Highly organized, effective at multitasking, adept at juggling changing priorities and able to prioritize.
Proven ability to work both independently and also as part of a collaborative team.
Excellent communication skills, both oral and written.
Strong interpersonal skills, including tact, diplomacy, and flexibility.
Demonstrated ability to interact effectively with diverse groups. Demonstrated skill in establishing and maintaining effective working relationships with personnel at all levels.
SPECIAL CONDITIONS OF EMPLOYMENT
Must be willing to work with rodents (mice, rats) in an animal-related research environment.
Must be willing to work with human cells and complete all appropriate associated training.
Must be willing to work independently and must have a flexible schedule to support research needs.
Successful completion of the Institute’s background investigation.
Must be willing to sign a confidentially agreement
This position requires constant adjusting focus, seeing, and working inside.
Equal Opportunity Employer/Protected Veterans/Individuals with Disabilities
The contractor will not discharge or in any other manner discriminate against employees or applicants because they have inquired about, discussed, or disclosed their own pay or the pay of another employee or applicant. However, employees who have access to the compensation information of other employees or applicants as a part of their essential job functions cannot disclose the pay of other employees or applicants to individuals who do not otherwise have access to compensation information, unless the disclosure is (a) in response to a formal complaint or charge, (b) in furtherance of an investigation, proceeding, hearing, or action, including an investigation conducted by the employer, or (c) consistent with the contractor’s legal duty to furnish information. 41 CFR 60-1.35(c)
Salk scientists are dedicated to innovative biological research. The Salk Institute consistently ranks among the leading research institutions in the world for its faculty's contributions and the impact of their findings. Focused both on discovery and on mentoring future generations of researchers, Salk scientists make groundbreaking contributions to our understanding of cancer, aging, Alzheimer's, diabetes and infectious diseases by studying neuroscience, genetics, cell and plant biology, and related disciplines. "We lead biological research. We prize discovery. Salk is where cures begin."Overlooking the Pacific Ocean in La Jolla, the Salk Institute offers a warm, collegial and collaborative work environment, where employees enjoy a generous benefits program. Social activities on campus encourage interaction as a community.