UCSD Layoff from Career Appointment: Apply by 05/04/21 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 05/13/21. Eligible Special Selection clients should contact their Disability Counselor for assistance.
Maintain and oversee laboratory for all safety regulations, maintenance of all equipment and computers, and provide technical assistance for in vivo and in vitro techniques. Independently perform various mammalian cell culture techniques such as cell propagation, contaminant testing, cryopreservation, and performing flow cytometry to analyze cell surface protein expression. Independently conduct procedures with mice, such as breeding colony management, drug dosing, and tissue harvest. Provide technical assistance for experiments involving mouse surgeries and cancer models. Maintain confocal, brightfield and fluorescence microscopy suite, providing troubleshooting repairs, imaging and training.
Provide technical assistance for specialized molecular biology techniques such as polymerase chain reaction and agarose gel electrophoresis for mouse genotyping. Perform tissue analysis including sectioning fixed tissues, histological staining, and immunohistochemistry. Independently perform protein biochemistry techniques including protein quantification, SDS PAGE, immunoprecipitation, and Western blotting. Independently perform experiments to evaluate gene expression, including RNA isolation, RT-PCR, real-time qPCR. Image tissues and cells to document experiments using multiple techniques, and provide the results in a clear and organized manner. Maintain and prepare accurate records of all experiments. Collaborate with lab personnel to document experiments, analyze data, and prepare projects for publication.
Strong organizational skills to order and maintain lab equipment and supplies.
Proven experience working with chemical and biohazardous materials. Experience working with sterile techniques.
Proficient experience working with computer applications such as Word, Excel and PowerPoint. Experience Excel to analyze data, store results and compute raw data. Experience with Internet searches and software analysis capabilities.
Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment, as well as to effectively and accurately document research procedures. Ability to read, comprehend and discuss research materials.
Ability to adhere to established UCSD guidelines for safety and environmental issues.
Detail-oriented. Ability to work with little supervision. Ability to follow verbal and/or written instructions. Ability to follow-up on previously begun procedures. Ability to accurately record data and enter into appropriate medium.
Advanced theoretical and working knowledge of molecular biology and immunology as typically attained by a Bachelor's degree, or an equivalent combination of education and experience.
Experience handling and working with small animals, including experience with anesthesia and euthanasia.
Experience using light, fluorescence, and confocal microscopy techniques to visualize cells and processed tissue sections.
Experience performing PCR and agarose gel electrophoresis.
Experience working with basic laboratory equipment such as centrifuges, spectrophotometers, balances, pH meters, autoclaves and electrophoresis equipment.
Advanced experience performing DNA characterization techniques using electrophoretic analysis.
Knowledge and experience to teach principal laboratory operations, theories, molecular biology techniques, tissue culture operations and techniques, and laboratory safety procedures and policies to lab members.
Experience in providing work direction and assisting supervisor in maintaining an efficient laboratory research team.
Employment is subject to a criminal background check.
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