The Department of Microbiology & Immunology is seeking a full-time laboratory Technician B to provide technical support for research on bacterial dormancy in the Dworkin Laboratory.
The Dworkin laboratory is interested in the mechanisms used by quiescent cells to survive stressful conditions, such as nutrient limitation with particular attention on how these strategies affect antibiotic sensitivity including the emergence of tolerance (???persisters???). We focus on the down-regulation of protein synthesis, with most recent work examining the nucleotide ppGpp and the protein Hibernation Promoting Factor which induces ribosome dimerization. Our work employs a range of techniques, ranging from genetics and physiology to in vitro biochemistry and single molecule biophysical studies.
Responsibilities will include preparation of growth media and stock solutions, ordering replacement supplies and maintaining laboratory equipment. In addition, the technician B will assist members of the laboratory to perform experiments including genomic DNA isolation, PCR, flow cytometry, and microscopy. The technician B will be required to keep detailed records of all activities.
Bachelor's degree in chemistry, biology or physics or equivalent in education, training and experience.
Ability to set up and conduct experiments or activities according to established protocols
Ability to record and maintain data and activities
Knowledge of related basic laboratory techniques
Equal Opportunity Employer / Disability / Veteran
Columbia University is committed to the hiring of qualified local residents.
Internal Number: 515015
About Columbia University
Columbia University is one of the world's most important centers of research and at the same time a distinctive and distinguished learning environment for undergraduates and graduate students in many scholarly and professional fields. The University recognizes the importance of its location in New York City and seeks to link its research and teaching to the vast resources of a great metropolis. It seeks to attract a diverse and international faculty and student body, to support research and teaching on global issues, and to create academic relationships with many countries and regions. It expects all areas of the university to advance knowledge and learning at the highest level and to convey the products of its efforts to the world.