Lead projects focused on characterizing the role of the Mycobacterium tuberculosis ESX-5 Type VII secretion system in bacterial physiology and pathogenesis. The candidate will use a variety of experimental techniques including construction of genetically modified M. tuberculosis strains, protein extraction and Western blotting, gene expression analysis, macrophage and mouse infection models, fluorescence microscopy and flow cytometry. The candidate will work independently to execute and troubleshoot experiments and analyze the results. They will analyze the primary literature in the field to develop new hypotheses and lines of experimental inquiry. The candidate will participate in weekly joint lab meetings with other TB research labs. The candidate will present at least one formal seminar per year locally or at relevant conferences/symposia (local, regional or national).
20% Writing scientific manuscripts and grants applications:
Be responsible for writing drafts of manuscripts to communicate experimental results. They will serve as primary author or co-author of manuscripts. They will work with the PI on planning and writing proposals for extramural research funding.
5%: Training/supervision of graduate students, technicians and/or undergraduates:
Assist in the technical training and supervision of graduate students, technicians and undergraduates in the lab. This includes hands-on training and assisting with data analyses and troubleshooting experiments.
A Ph.D in microbiology, molecular biology or related discipline
Advanced knowledge of microbiology and molecular biology techniques: cloning, bacterial genetics, Western blotting, gene expression analysis, etc.
Previous experience with bacterial infection models: cell culture or animal
One or more first author research publications in peer-reviewed journals
Excellent written and oral communication skills
Prior research experience with mycobacterium
Prior research experience in a BSL-3 facility
Experience with macrophage cell culture
Experience with mouse infection models
Experience with fluorescence microscopy or flow cytometry
Internal Number: 341837
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