This position will remain open until a successful candidate has been identified.
UCSD Layoff from Career Appointment: Apply by 07/14/21 for consideration with preference for rehire. All layoff applicants should contact their Employment Advisor.
Special Selection Applicants: Apply by 07/23/21. Eligible Special Selection clients should contact their Disability Counselor for assistance.
The Department of Pathology at UC San Diego aims to achieve the highest standards in patient care, research and education. The Department provides diagnostic pathology services in a wide variety of subspecialties with highly trained subspecialists who are recognized for their expertise. The Department's research programs are diverse and interface with a wealth of basic and translational research programs that are a hallmark of UC San Diego.
This Lab Assistant will work in a laboratory investigating the role of the microbiota in modulating immune responses during health and disease. Utilizing the combination of genetically engineered mice maintained under germ-free or defined microbial communities, our lab is able to explore the multifactorial contributions to disease pathogenesis. Under supervision, the applicant will assist in performing experiments in immunology and characterize mucosal immune response to gut microbes in mouse models of disease. These experiments include monitoring and treatment of laboratory animals, tissue processing for flow cytometry, histological assessment, and downstream immunological assays.
Other responsibilities include tissue culture, cell isolation and other cell-based assays, and basic cell and molecular biology techniques. Additionally, this applicant will assist in performing laboratory maintenance and administrative duties including ensuring compliance with IACUC and EH&S requirements, ordering and tracking of supplies, data organization, teaching basic laboratory techniques and preparing data for presentation.
Two (2) years of laboratory experience or two (2) years of college including courses in the natural, physical or social sciences and one (1) year of laboratory experience, or an equivalent combination of education and experience; and knowledge and abilities essential to the successful performance of the duties assigned to the position.
Theoretical knowledge in immunology or a related biomedical science.
Experience and ability to initiate and maintain mouse experiments including treatment and observation for disease. Experience with mouse handling, breeding, genotyping, anesthesia, surgeries, injections/treatments, and tissue harvest.
Proven experience and ability in multi-parameter flow cytometry (8+ colors), including panel design, staining, compensation, troubleshooting, analysis, and preparation of figures for publication.
Experience and demonstrated ability in extracting DNA/RNA, PCR, RT-PCR, qPCR, primer design, analysis and troubleshooting.
Experience and ability to initiate and maintain animal experiments multiple different eukaryotic cell lines including freezing, thawing, storage, and plating cells in preparation for experiments. Experience with sterile techniques, trouble shooting of contamination problems, and care of incubators and liquid nitrogen freezers.
Demonstrated experience in protein chemistry/purification, gel electrophoresis and transfers, various gel and membrane stains, ELISAs, western blots, and densitometry and analysis on resulting data.
Thorough knowledge of microbiological techniques, including culturing of aerobic/anaerobic bacteria, aseptic technique, and generating glycerol stocks with documentation.
Excellent communication and interpersonal skills over a wide range of situations and individuals needed to interact professionally and effectively in the work environment in person, on phone, and in writing to diverse population; including persons from foreign countries, the business community, academicians, students and staff with a high degree of diplomacy, mature judgment, tact, and flexibility.
Knowledge of proper formats, grammar, and spelling in English and proofreading ability sufficient to compose, edit, and publish professional correspondence as well as effectively and accurately document research procedures. Ability to read, comprehend, discuss, and present research materials.
Knowledge of bio, chemical, and radiation safety techniques and practices. Ability to follow established Safety and Biohazardous Material Guidelines and implement measures to ensure compliance and maintain associated records.
Strong organizational skills to order and maintain la
UC San Diego is an academic powerhouse and economic engine, recognized as one of the top 8 public universities by U.S. News and World Report. Innovation is central to who we are and what we do. Here, students learn that knowledge isn't just acquired in the classroom - life is their laboratory. UC San Diego's rich academic portfolio includes six undergraduate colleges, five academic divisions and five graduate and professional schools. The university's award-winning scholars are experts at the forefront of their fields with an impressive track record for achieving scientific, medical and technological breakthroughs.