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The Department of Microbiology has an outstanding opportunity for a Research Scientist 1.
The Sherman Lab is focused on gene regulation, systems biology and pathogenesis of tuberculosis, and developing novel drugs, diagnostics and vaccines to combat this infection
Perform standard molecular biology procedures and assays: •Isolate genomic DNA and RNA from cultures; •Isolate bacterial chromosomal and plasmid DNA; •Perform general biochemical assays, including spectrophotometry, spectrofluorometry, gel electrophoresis, chromatography; •Perform bacterial growth inhibition and mammalian cell infection assays; prepare solutions for experiments; obtain accurate results and carry out experiments in a timely manner; •Identify methodological problems in research protocol and implement modifications to optimize experimental results, as authorized by the PI; •Learn new techniques with supervision. Work in coordination with team members to design and execute experimental protocols; •Assist in directing projects by contributing intellectually to design and direction of research; •Meet with team members to discuss experiment design; •Determine procedures and techniques needed to accomplish experimental goals; •Implement plan, including determination of reagents needed and their preparation.
Analyze scientific data: •Develop and maintain databases; •Interpret results utilizing statistical or computer analysis; •Ensure data are entered accurately; •Report summary of results to PI.
Assist in general maintenance of the laboratory and lab systems: •Prepare solutions, buffers, and reagents; •Clean glassware as needed; •Order/maintain supplies for day-to-day operation of the lab; •Assist in maintenance/repair of laboratory equipment. Work in biosafety level III (BL-3) laboratory in addition to standard BL-2 lab.
May assist in animal experiments: •Assist in animal infections and tissue harvesting.
Adhere to standard work plans and continuously seek incremental improvement
•Bachelor's degree in biology, microbiology, biochemistry, immunology, molecular biology, or related field •Minimum of 1 year in research laboratory setting.
Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration.
•Knowledge of scientific discipline and applicable techniques and procedures used in a research lab setting. •Knowledge of basic principles of media preparation and sterile techniques. •Knowledge of software applications, proficiency using MS Office (Outlook, Word, Excel & Access), and ability to learn new software programs. •Excellent problem-solving skills. •Strong interpersonal skills. •Good laboratory techniques and record keeping skills. •Good organization skills, including attention to detail. •Data collection, analysis and summary skills. •Able to establish and maintain effective working relationships with a variety of people from diverse backgrounds. •Willingness and ability to learn new methods, approaches, and experimental techniques. •Able to move around different work sites to perform job duties. •Able to work with potentially hazardous chemical materials and biohazard samples
•Two years in research laboratory setting. •Knowledge of DNA analysis software and related computer techniques. •Able to work independently with minimal supervision. •Experience with isolation of RNA and drug exposure assays. •Able to recognize emergency situations and take appropriate action as defined in emergency procedures. •Experience in analyzing data using spreadsheets and statistical analysis. Research experience with biosafety level III pathogens and with animal infections helpful.
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