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Founded in 1861, the University of Washington is one of the oldest public institutions in the west coast and one of the preeminent research universities in the world. The University of Washington is a multi-campus university comprised of three different campuses: Seattle, Tacoma, and Bothell. The Seattle campus is made up of sixteen schools and colleges that serve students ranging from an undergraduate level to a doctoral level. The university is home to world-class libraries, arts, music, drama, and sports, as well as the highest quality medical care in Washington State and a world-class academic medical center. The teaching and research of the University’s many professional schools provide undergraduate and graduate students the education necessary toward achieving an excellence that will serve the state, the region, and the nation. As part of a large and diverse community, the University of Washington serves more students than any other institution in the Northwest.
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RESEARCH SCIENTIST SENIOR
University of Washington
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Posted: 13-Oct-21

Location: Seattle, Washington

Type: Full Time

Salary: Open

Internal Number: 197477

A higher degree of healthcare.

All across UW Medicine, our employees collaborate to perform the highest quality work with integrity and compassion and to create a respectful, welcoming environment where every patient, family, student and colleague is valued and honored.

The Department of Laboratory Medicine and Pathology serves as a regional resource for clinical laboratory services required for patient care, research and educational programs in laboratory medicine and pathology. The department now employs 900 people who work at the award-winning University of Washington Medical Center, Harborview Medical Center and many other clinical and research facilities in the area. The University of Washington (UW) is proud to be one of the nation’s premier educational and research institutions. Our people are the most important asset in our pursuit of achieving excellence in education, research, and community service. Our staff not only enjoys outstanding benefits and professional growth opportunities, but also an environment noted for diversity, community involvement, intellectual excitement and natural beauty.

Our Laboratory Medicine and Pathology department has an outstanding opportunity for a Full-time, (100%) RESEARCH SCIENTIST SENIOR

The employee will serve as a Senior Research Scientist in the Greninger Laboratory. Dr. Greninger oversees three laboratories, including clinical virology, virology clinical trials, and virology basic sciences, so the job can be fluid depending on the rigors at the time. This is an awesome and highly involved position desirous of an applicant with tremendous energy, combining wet-lab and dry-lab work in the area of emergent viruses. The work generally involves excellent molecular biology, biochemistry, virology, and informatics skills to oversee a multitude of projects associated with protecting humans from viral disease. This specific position will involve design and oversight of research involving viral discovery, construction of metagenomic next-generation sequencing libraries and analysis of said libraries, and understanding of antiviral resistance pathways to known viruses as well as determining antiviral mechanism.  Viral characterization work will involve work in viral replication, viral-host protein-protein interactions, drug efficacy, viral evolution, and viral genome sequencing. Responsibilities include constructing and teaching others on the generation of next-generation sequencing libraries, analyzing sequencing data, visualizing data and programming figures, drafting of manuscripts and grants for submission, combined with follow-on wet-lab experiments involving gene synthesis, pseudovirus neutralization and entry assays, and protein purification.

RESPONSIBILITIES
  • Oversight of creation and optimization of metagenomic next-generation sequencing libraries for unbiased pathogen detection and viral genome recovery. 
  • Genome finishing of novel viruses, including optimization of high-throughput methods.
  • Development of next-generation sequencing analysis pipelines for taxonomical analysis and viral genome visualization.
  • Negotiate with vendors for reagents and instrumentation needed for expanded next-generation sequencing capacity in associated laboratories
  • Review technical development plans and assist in planning for annual division research and development technical goals and objectives
  • Interact with laboratory clients and collaborators related to aspects of technical development.
  • Development of phage display serological profiling tools to investigate high-resolution interrogation of serological response, including creation of analysis tools.
  • Perform high-level viral culture in novel culture models and reverse genetics work, analyze data compile results and draft reports for incoming research awards and contracts
  • Supervise and analyze quantitative viral load determination using RT-qPCR, qPCR, ddPCR, including optimization of new assays
  • Supervise performance of molecular procedures including but not limited to real-time PCR, Sanger sequencing, PCR product detection DNA sequencing and next generation sequencing;
  • Host and teach scientists in above techniques from Kenya, Democratic Republic of Congo, Tanzania, Peru, Brazil, Bangladesh, Senegal, Vietnam, India, Cameroon, Indonesia, Thailand and possible travel to these countries.
  • Perform work accurately, precisely and meet test completion deadlines;
  • Maintain complete, accurate, legible, secure records.  Thoroughly document any modifications using appropriate document and version control.
  • Analyze experimental data and report to the Director and Lab Supervisor; determine what modifications and follow-up experiments are necessary to generate relevant data;
  • Identify long and short-term goals; initiate research program/projects;
  • Contribute technical knowledge when initiating new projects to determining feasibility of goals and objectives of the study;
  • Contribute data and analyses for grant submissions;
  • Perform preliminary experiments for assay development;
  • Work with R, python, Geneious, Excel, and bash command line;
  • Accommodate schedule changes to cooperatively accomplish required daily work, coordinate with Laboratory supervisor/manager;
  • Collaborate with research team to create opportunities and ancillary research projects to enhance technical assay methodology or content through the expansion of existing or development of new projects;
  • Review the scientific process of each research project, analyze issues, understand scientific procedures and statistical analysis; report data using graphs and charts;
  • Provide ongoing feedback; identify development opportunities and actively solicit input on work methods;
  • Prepare manuscripts for publication;
  • Perform other duties as required.
  • Review high-complexity quality control data for Virology Division laboratories.
  • Participate, as needed, in teaching of undergraduates, graduate students, residents, and fellows.
  • Participate in research and scholarship related to the Virology Division leadership.

    REQUIREMENTS
  • PhD in Biology, Molecular Biology, Microbiology or closely related field AND 
    six years experience in related area or equivalent combination of education/experience in collaborative project design, and understanding of genomics and/or viral biology.

    ADDITIONAL REQUIREMENTS
  • Strong written and communication skills.
  • Ability to problem-solve and troubleshoot.

    An equivalent combination of education and experience may substitute for stated requirements.

    DESIRED
  • Proficient at R, python, and/or bash.
  • Data analysis skills with basic statistical knowledge.

    UW Medicine includes Harborview Medical Center, UW Medical Center - Montlake, UW Medical Center - Northwest, Valley Medical Center, UW Neighborhood Clinics, UW Physicians, UW School of Medicine, and Airlift Northwest.