Summary of Position Dedicated researcher to join an accelerating and highly collaborative research group in the University of Minnesota Department of Biochemistry, Molecular Biology, and Biophysics. The researcher will assist experienced scientists in carrying out large-scale molecular biology workflows including cloning, mutagenesis, and protein engineering.
The researcher will assist experienced scientists in performing high-throughput biochemical assays of a wide range of complexities, and assist in assay development for basic hypothesis driven biochemical and cellular discovery projects.
The researcher will assist in the development of improved therapeutics for treating a wide range of diseases including diseases of the skeletal muscle and heart.
The researcher will keep clear and extensive records of scientific work and perform basic lab maintenance including reagent stock management and ordering. The researcher will participate in weekly laboratory meetings to discuss ongoing research with the research team.
30% Molecular Biology and Cell Culture DNA & RNA isolation and sequencing; Nucleic acid gel electrophoresis; PCR of diverse DNA templates; PCR primer design and optimization; PCR reaction optimization; Plasmid design including design of expression plasmids for recombinant protein expression in bacteria, insect, and mammalian cells; Construction of plasmids of varying size using a variety of methods including conventional restriction enzyme ligation approaches, Gibson Assembly and/or Golden Gate Cloning: Plasmid mutagenesis; Generation of DNA libraries; Culture and maintenance of mammalian cell lines.
30% Protein biochemical and biophysical assays Elisa, Western blotting, optical spectroscopy, protein thermal stability, protein-protein interaction characterization.
30% Recombinant Protein Expression and Purification Protein expression in Bacteria and Mammalian cells. Protein purification using affinity tags. Protein purification using chromatography systems.
10% General Laboratory Maintenance Inventory and organize laboratory materials,Maintain general working order of laboratory, Assist in ordering materials for laboratory work.
All required qualifications must be documented on application materials
BA/BS or a combination of related education and work experience to equal four years in any of the following areas: Microbiology, Chemistry, Biochemistry, Biology, Pharmacology, or related field.
Experience in Mammalian Cell culture, or Protein expression and purification.
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.