The histology/immunohistochemistry technician will assist veterinary pathologists to diagnose animal diseases by preparing specimens for histology, histochemistry (special stains), Immunohistochemistry (IHC), and in situ hybridization (ISH [RNAScope]) slides for microscopic examination by carrying out all phases of trimming and processing of tissues submitted to the Louisiana Animal Disease Diagnostic Laboratory (LADDL).
20%- Trim formalinized necropsy, biopsy, mail-in tissue specimens and deer tissues for chronic wasting disease (CWD) testing, and place them in labeled cassettes. Match all necropsy, biopsy and mail-in tissues with corresponding paperwork. Perform measurements of specimens, tissues, structures, and lesions as needed. Record measurements as instructed by the pathologists. Keep records by entering data in logbooks and computer. Load tissues into the automated processor at the end of the day
20%- Process and embed tissues (paraffin and frozen) and prepare microscopic slides for LADDL pathologists or researchers. Essential functions include preparing paraffin tissue blocks and OCT embedding and frozen sectioning (cryotomy) of specimens from necropsy, mail-in, biopsy and research. In addition to paraffin infiltration and embedding of the tissues, cut tissue blocks using microtome, stain (i.e., Hematoxylin and Eosin) and coverslip slides.
40%- Perform manual and automated IHC staining according to established protocols using both manual and automated instrument procedures. This includes IHC staining of deer tissue for prions (i.e., causative agent of CWD). Check the quality of the stain with appropriate controls. Label slides with case numbers and delivers the slides to pathologists. Keep records by entering data in logbooks and computer. Perform primary and secondary antibody titrations, trouble shooting, and development and validation of new IHC and immunofluorescence (IF) protocols for cellular markers and antigens (e.g., bacterial, viral, parasitic and prions). Maintain a bank of appropriate positive and negative control tissues for each assay. Establishment of published ISH/RNAScope protocols and development and validation new assays.
5%- Perform billing, ordering and maintain reagent inventory for the IHC/ISH section. Keep records by entering data in logbooks and computer. Operate and maintain equipment, maintain chemical inventory and adhere to LSU Biological Safety Policies of Environmental Health and Safety, as well as AAVLD quality assurance standards by maintaining SOPs.
5%- Essential functions will include to follow laboratory safety procedures, operate histology and IHC equipment safely and correctly, perform daily and weekly maintenance of equipment, maintain clean laboratory environment, make sure all formalinized tissues and other chemicals are stored properly, file paraffin and frozen blocks and slides, and prepare lab reagents and complete other duties assigned by the pathology or IHC section head or the lab director.
5%- Provide backup support (floater) for other histology technicians when needed. This includes helping with paraffin infiltration and embedding of the tissues, cut tissue blocks using microtome, stain (i.e., Hematoxylin and Eosin and special) and coverslip slides. Label slides with case numbers and delivers the slides to pathologists.
5%- Maintain highest professional standards, promote excellent customer service to internal and external constituents and commit to providing excellent diagnostic service to the LADDL clients. Expand technical and professional knowledge through training, classes, and seminars. Perform other duties assigned by the pathology or histology section head or the lab director.
Bachelor's degree or Master's degree in a related field
Specific Degree- Biological Science, Chemistry, Medical Technology or related field
Years of Experience- 2-4 years experience working in research or diagnostic laboratory
Specific Experience- Working knowledge of IHC theory and hands on IHC performance. Hands on immunofluorescence and ISH performance. Tissue embedding, microtomy, tissue section staining, reagents preparation, handling of hazardous waste and trimming of formalized tissue specimens is required.
-Education Level- Master's
-Specific Degree Required- Biological Sciences Chemistry, Medical Technology or related field, or a Master's degree in a related field
-Years of Experience- 2 - 4 years experience working in human or veterinary histology/IHC laboratory, research or diagnostic laboratory
-Specific Experience-Working knowledge of histology, histochemistry, or IHC theory and hands on IHC performance. Hands on immunofluorescence and ISH performance. Tissue embedding, microtomy, tissue section staining, reagents preparation, handling of hazardous waste and trimming of formalized tissue specimens is required.
-ASCP Certification or QIHC Certification
Special or Physical Qualifications:
Required to be capable of lifting more than 50 pounds, stand or walk for prolonged periods, able to reach and/or climb a ladder, kneel or sit on the floor, bending, squatting, and/or twisting. Must be able to distinguish between different colors accurately and have near-vision acuity at 20 inches or less. Per PS 18- This position is a part of the Emergency Response Personnel - these personnel are linked with the hospital during any emergency response event and are expected to report in and perform as outlined in the emergency response plan.
A copy of your transcript(s) may be attached to your application (if available). However, original transcripts are required prior to hire.
Please provide three professional references including name, title, phone number and e-mail address.
Additional Position Information:
Background Check - An offer of employment is contingent on a satisfactory pre-employment background check.
Benefits - LSU offers outstanding benefits to eligible employees and their dependents including health, life, dental, and vision insurance; flexible spending accounts; retirement options; various leave options; paid holidays; wellness benefits; tuition exemption for qualified positions; training and development opportunities; employee discounts; and more!
LSU is an Equal Opportunity Employer:
LSU believes diversity, equity, and inclusion enrich the educational experience of our students, faculty, and staff, and are necessary to prepare all people to thrive personally and professionally in a global society. We celebrate diversity and are committed to the principles of diversity and inclusion. We actively seek and encourage qualified applications from persons with diverse backgrounds, cultures and experiences. To learn more about how LSU is committed to diversity and inclusivity, please see LSU’s Diversity Statement and Roadmap. Persons needing accommodations or assistance with the accessibility of materials related to this search are encouraged to contact the Office of Human Resource Management (email@example.com).
COVID-19 Vaccine Protocol:
LSU requires the COVID-19 vaccine for all students, faculty, and staff or mandatory monthly testing. New employees must either submit proof of vaccination within three (3) days of their official start date, otherwise they will be entered into the mandatory monthly testing protocol beginning in November 2021
HCM Contact Information:
Questions or concerns can be directed to the LSU Human Resources Management Office at 225-578-8200 or emailed HR@lsu.edu
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