The Columbia Stem Cell Initiative (CSCI) Stem Cell Core, under the supervision of its director Dr. Barbara Corneo, is seeking an outstanding Associate Research Assistant to carry out core facility tasks and assist with assigned research projects on cellular reprogramming in the laboratory of Dr. Passegué, the director of CSCI.
Reporting to the Stem Cell Core director, the Associate Research Assistant will carry out independently the daily activities of the core facility.
Derives fibroblasts from skin biopsies and PBMC from blood samples.
Derives, maintains and distributes induced pluripotent stem cell lines.
Characterizes new lines by standard molecular biological techniques (including DNA/RNA extraction, PCR, qPCR), by immunophenotypic analysis (including immunostaining and flow cytometry) and by functional assays (in vitro differentiation).
Performs quality control of cell lines, including microbiological screening.
Performs differentiation of iPSC into various progenies.
Performs gene editing services, including CRISPR-Cas9 mediated genome modification of iPSCs and other cell lines.
Instructs others in tissue culture techniques for iPSC maintenance/differentiation and gene editing.
Assist in the organization and teaching of training workshops for researchers interested in learning techniques relevant to iPSC derivation, culture, differentiation and gene editing.
Performs laboratory maintenance and management, including maintaining and cleaning equipment, interfacing with vendors, ordering and keeping an adequate inventory of laboratory supplies, tracking core usage and invoicing participating laboratories.
Assists in the preparation of progress reports, grant applications, and manuscripts relevant to the core's activities.
Maintains accurate records.
Under the oversight of Dr. Passegué, the Associate Research Assistant will execute experiments and research tasks on assigned research projects, and help to plan future research strategy. Scientific growth will be encouraged, and relevant research projects involving cellular reprogramming approaches will be assigned as needed.
PhD in biology or related field. Minimum 2 year of experience with iPSC derivation, cell reprogramming and directed differentiation methods, and CRISPR-Cas9 gene editing technology. Previous experience with primary cell cultures and sterile laboratory technique is required. Exceptional organizational and record keeping skills and excellent attention to detail are an essential component of this position. Ability to work proactively and in a collaborative, team-oriented environment. Some weekend flexibility will be required. Computer experience is necessary.
The applicant should be self-motivated and possess excellent communication and interpersonal skills. He/she should also have outstanding organizational skills, the ability to multitask, and be able to work independently as well as within a team. Success in this position will require proactivity, attention to detail and willingness to learn.
Working knowledge of Microsoft Office suite, as well as software related to lab activities, such as Prism, Illustrator, and statistical software.
Please find more information on CSCI, the Stem Cell Core and the Passegué lab here:
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