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Research Assistant II - Stem Cell Core Facility
This position will involve the execution of standard laboratory procedures with human somatic cell and human pluripotent cell lines, as well as other experimental procedures. The Research Assistant II will assist in generating custom knockin and knockout iPS cell models using CRISPR genome editing. Duties involved in the workflow include cell culture/stem cell culture, transfection, cryopreservation of cells, genomic DNA isolation, PCR, gel purification, cloning, DNA sequence analysis, and gel electrophoresis. Procedures and techniques may include but are not limited to: derivation of somatic cells from biopsy materials, maintenance, banking, and characterization of cell lines, transduction and transfection of cell lines, virus production


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