The Nguyen Lab is currently developing small molecule probes, biochemical, cell biological and genetic approaches to investigate the underlying DNA damage response mechanisms in cancers. One area of active research is to investigate mechanisms of sensing and resolving R-loops in cancers to provide molecular insight for the development of new targeted cancer therapeutic approaches. Research technicians are responsible for performing standard bench-level laboratory experiments in support of scientific research.
Job duties include: 1) 90% Laboratory research • Perform experiments at the bench level to support investigative work (DNA/RNA isolation and analysis, protein isolation and SDS-PAGE analysis, standard molecular biology techniques) • Prepare and maintain laboratory reagents, stocks, cultures or cell lines using sterile techniques • Perform and modify documented protocols and assays • Maintain laboratory records and compile data on experiments and assays • Interpret and report on experimental results • Perform statistical analysis of test results and prepare written reports on work performed • Participate in lab meetings and journal clubs • May supervise other trainees in the laboratory.
2) 10% Lab management • Organize, track, and order lab supplies and reagents • Organize and maintain laboratory protocols, cryopreservation of cell lines, bacterial and plasmid stocks. • Organize and maintain up-to-date laboratory safety protocols. • Keep track of lab personnel training records.
All required qualifications must be documented on application materials
Bachelors degree in biochemistry, biology, pharmacology, or related fields; or a combination of related education and experience to equal four (4) years.
Must have scientific knowledge in the area of research.
Have at least 1-2 years of independent research experience beyond school lab course experiences is preferred.
DNA manipulation, generation, purification, and analysis: PCR, DNA cloning, bacterial transformation, and plasmid isolation and purification. DNA analysis such as DNA gel electrophoresis and analyzing DNA sequencing results is preferred.
Western blot techniques: SDS-PAGE gel electrophoresis and western blot.
Routine mammalian cell culture, transfection using sterile techniques is preferred.
A successful candidate will have scientific curiosity, a high level of self-motivation, the ability to take instruction and correction and to follow directions, good organizational skills and the ability to work and think independently. The candidate must have a track record of reliability and good verbal and written communication skills. This is an ideal position for a recent graduate who wants to gain an intense research experience before pursuing graduate school.
The University of Minnesota, founded in the belief that all people are enriched by understanding, is dedicated to the advancement of learning and the search for truth; to the sharing of this knowledge through education for a diverse community; and to the application of this knowledge to benefit the people of the state, the nation, and the world.