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The Department of Microbiology has an outstanding opportunity for a Research Scientist /Engineer 1.
The research that the Research Scientist/Engineer 1 is engaged in are studies which develop and test novel vaccines and immunomodulators for the therapy or prevention of infectious diseases with an emphasis in Influenza and SARS-CoV-2 research. Projects include, but are not limited to, investigating the role of virus specific T cell responses and antibody responses in viral control, testing immunogenicity and efficacy of vaccines and therapeutics in nonhuman primate and murine models for HIV, ZIKV, influenza, Hepatitis B, SARS-CoV-2 (and others); investigating mechanisms underlying mucosal dysfunction induced by lentivirus infections; development and treatment of the latent viral reservoir during antiretroviral drug therapy, novel therapeutic interventions to treat HIV disease pathogenesis.
Laboratory Bench Work: •Tissue culture techniques; preparation of necropsied tissue; •tissue processing including isolation of lymphocytes from lymph nodes, jejunum, vagina, rectum, blood, colon, & bronchio-alvelolar lavage using Percoll, Ficoll, and other lymphocyte separation techniques; cryopreservation of cells; •support for immunophenotyping & intracellular cytokine staining assays, ELISA; •flow cytometric analysis of cell mediated immune responses
Support projects at BSL2/ABSL2 & the high-contaiment BSL3/ABSL3 facility for mice conduct/support: •plaque assays, PRNT assays; •HAI assay, mouse handling, mouse bleeds, infections, weighing, tissue sampling, anesthesia via isoflurane gas or ketamine/xylazine administration, anesthesia recovery monitoring, humane euthanasia of mice, ELISA, flow cytometric sample preparation and staining, therapeutic drug candidate administration, experimental vaccine administration, tissue culture; •Luminex-based multiplex assays; •qPCR and other PCR-based methodology; •nucleic acid extraction.
General Laboratory Maintenance and Support: •shipping category B samples to collaborators; •receive/store shipments from collaborators/vendors; •monitor supply inventory and alert purchasing team when low; •coordinate broadly with team members to prepare equipment/supplies in support of tissue processing/assays; •maintain electronic laboratory notebook, regularly report experimental results to manager; •present results in lab meetings; •perform biosafety 2+ practices; •other duties as assigned.
REQUIRED •Bachelor's degree in biochemistry, microbiology, or other biological science •Minimum of one year of job-related experience •Experience and knowledge in BSL2-3 practices; •Experience with specimen preparation from nonhuman primates; •Experience with cryopreservation of nonhuman primates specimens; •Working knowledge and experience cell counting using a Nexcelcom cell counter, significant experience in processing tissues from nonhuman primates and mice including purifying lymphocytes from lung, spleen, liver, bronchioalveolar lavages, lymph nodes, GI tract, & reproductive tract tissues; •Experience in long-term tissue fixation in formalin & ethanol, OCT molds/cassettes; •Cell/tissue culture methodology; •HAI Assay; ELISA; ELISPOT; ICS; Luminex/multiplex; qPCR/Rt-qPCR; PCR-based methodology; nucleic acid extraction. •Prior experience with organizing and restocking laboratories; •Autoclaving, aliquoting of reagents; •Experience acquiring samples on a BD LSR II flow cytometer and BD Symphony flow cytometer; •Demonstrated ability to work as part of a team and adapt to different tasks. •Mouse handling and sampling. •Excellent working knowledge with data analysis, inventory, and presentation software including MS Excel, Powerpoint, FACDiva, Sample retrieval and shipment to collabroators
Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration.
DESIRED •Prior experience with analysis of CD4+ and CD8+ T cell responses in nohuman primate specimens; •Experience with data analysis software including Flow-Jo, Prism; •ELISpot assay performance and analysis; performing various surface, and intercellular cytokine stains for FACS; •Ability to make independent judgments that will promote efficiency and add value to laboratory's research; •Strong organizational skills including ability to work efficiently with competing priorities and deadlines; Packing and shipment of precious Category A research samples; •qPCR; •Mouse handling
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