The Great Lakes Bioenergy Research Center (GLBRC) is a U.S. Department of Energy-funded Bioenergy Research Center led by the University of Wisconsin-Madison. With Michigan State University and other partners, we are developing approaches to generate biofuels and bioproducts through economically viable and environmentally sustainable technologies. The successful candidate will work in the Sato lab as part of GLBRC's Field-to-Fuel Optimization team. The central goal of the Sato Lab is to engineer microbial organisms that can tolerate harsh industrial conditions that impede effective and efficient conversion of carbohydrates from plant biomass to biofuels.
The primary responsibilities of this research specialist position are to perform and support lab research aimed at investigating new ways to genetically engineer S. cerevisiae yeast for improved cellulosic biofuel production. The research specialist will work in a team to: - Genetically engineer yeast for improved fermentation of plant-derived sugars into biofuels by using homology and CRISPR/Cas9-driven approaches. - Develop CRISPR/Cas9 perturbation (e.g., CRISPRi, CRISPRa) libraries in yeast and characterize for genetic modifications that impact cellular fitness and biofuel production - Amplify DNA barcodes by PCR of plasmid DNA from yeast strains, purify and sequence using Illumina platforms - Perform fermentation experiments with engineered and evolved strains to compare growth in various media (hydrolysates) derived from plant biomass. - Assist in preparing microbial media, reagents, and supplies as well as assisting in the organization and maintenance of the lab. - Present research progress and results in regularly scheduled meetings.
Develops, designs, and conducts one or more research projects and organizes daily operation of projects and specialized research areas to ensure work meets established objectives under moderate supervision.
50% Conducts research experiments according to established research protocols with moderate impact to the project(s). Collects data and monitors test results
15% Operates, cleans, and maintains organization of research equipment and research area. Tracks inventory levels and places replenishment orders
15% Reviews, analyzes, and interprets data and/or documents results for presentations and/or reporting to internal and external audiences
10% Participates in the development, interpretation, and implementation of research methodology and materials
5% Provides operational guidance on day-to-day activities of unit or program staff and/or student workers
5% Performs literature reviews and writes reports
Institutional Statement on Diversity:
Diversity is a source of strength, creativity, and innovation for UW-Madison. We value the contributions of each person and respect the profound ways their identity, culture, background, experience, status, abilities, and opinion enrich the university community. We commit ourselves to the pursuit of excellence in teaching, research, outreach, and diversity as inextricably linked goals.
The University of Wisconsin-Madison fulfills its public mission by creating a welcoming and inclusive community for people from every background - people who as students, faculty, and staff serve Wisconsin and the world.
Required Bachelor's Degree Bachelor's Degree required and/or Master's degree preferred in microbiology, genetics, molecular/cell biology, biochemistry, bioengineering, chemical engineering or relevant field.
Required minimum number of years and type of relevant work experience: - 2 years of post Bachelor's degree laboratory research experience including experience with microbiology, molecular and cell biology, and biochemistry techniques. - Experience in genetic and molecular biology approaches with microbes. - Specific experience with microbial culturing and sterile techniques, RNA and DNA purification, CRISPR/Cas9-based genetic engineering or gene perturbation (e.g., CRISPRi) and effectively working with others as part of a research team will be advantageous.
It is anticipated this position requires work be performed in-person, onsite, at a designated campus work location.
Appointment Type, Duration:
Minimum $39,400 ANNUAL (12 months) Depending on Qualifications Salary will be competitive depending upon experience and others in this title.
Please note that successful applicants are responsible for ensuring their eligibility to work in the United States (i.e. a citizen or national of the United States, a lawful permanent resident, a foreign national authorized to work in the United States without need of employer sponsorship) on or before the effective date of appointment.
How to Apply:
To apply for this position, click the Apply Now button (jobs.wisc.edu, search for 264667). Applicants will be prompted to upload a cover letter and resume. Cover letters should highlight experience, knowledge and skills as they relate to the listed qualifications for maximum consideration.
Emily Hickey email@example.com 608-890-2286 Relay Access (WTRS): 7-1-1 (out-of-state: TTY: 800.947.3529, STS: 800.833.7637) and above Phone number (See RELAY_SERVICE for further information. )
The University of Wisconsin is an Equal Opportunity and Affirmative Action Employer. We promote excellence through diversity and encourage all qualified individuals to apply.
Employment will require a criminal background check. It will also require you and your references to answer questions regarding sexual violence and sexual harassment.
The University of Wisconsin System will not reveal the identities of applicants who request confidentiality in writing, except that the identity of the successful candidate will be released. See Wis. Stat. sec. 19.36(7).
The University of Wisconsin–Madison has been a catalyst for the extraordinary since its founding in 1848. Our students, staff, and faculty partake in world-class education and conduct prolific research that solves real-world problems. Our guiding principle is the Wisconsin Idea, a commitment to outreach that creates a better future for our state, our nation, and the world. We value the contributions of each person and recognize that diversity is a source of strength, creativity, and innovation.