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The Department of Microbiology has an outstanding opportunity for a Research Scientist/Engineer 1 to join their team.
Pseudomonas aeruginosa is a major cause of lung infections in people with cystic fibrosis. Current treatments may have a limited effect which may lead to chronic infections. Biofilms, matrices that bacteria are forming, play an important role in the resistance to treatments. Our lab focuses on the mechanisms involved in the formation of biofilms, with a focus in Pseudomonas aeruginosa, aiming to advance our knowledge and therefore facilitate the development of new treatments. This role will provide support towards these projects. More particularly, this role studies bacterial signal transduction cascades and their ability to sense surfaces and initiate biofilm formation of Pseudomonas aeruginosa. The first part of this work involves basic bacterial cultivation techniques, molecular biological analysis, biochemistry, and microscopy skills. More particularly, it entails generating and verifying genetic mutations, verifying protein expression, and evaluating changes in bacterial behavior. This work also involves common cloning techniques, molecular biology techniques such as western blotting, and screening for common bacterial phenotypes. The second part of the job involves the use of fluorescence and confocal microscopy to evaluate the ability of Pseudomonas aeruginosa to adhere to surfaces and initiate signaling and biofilm formation. This involves the analysis of microfluidic devices for individual bacterial cells and biofilms. This work will be geared towards supplementing the experiments listed above.
DUTIES AND RESPONSIBILITIES
Information management (recording experiments and protocols, generating frozen stocks inventory, attending and presenting at regular lab meetings)
Generating and validating P. aeruginosa genetic mutants. This involves bacterial culturing, general cloning techniques, and molecular biology. The data will be gathered analyzed and organized in reports that can be shared with lab mates and collaborators.
Screening of P. aeruginosa surface sensing and biofilm phenotypes. This involves bacterial culturing and microscopy skills. The imaging of P. aeruginosa will require the organization and archiving of images.
Bachelor’s Degree in Biology, Microbiology or other pertinent science field.
Three month to one year of job-related experience.
Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration.
Basic molecular biology knowledge
The ability to clearly communicate and organize research efforts.
The ability to grow bacteria using aseptic technique.
The ability to do molecular biology including, but not limited to, polymerase chain reaction, designing primers, DNA electrophoresis, western blotting, and general microscopy skills.
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