Postdoctoral position in yeast genetics and molecular biology; DNA repair
Haber lab at Brandeis University
Location: Waltham, Massachusetts
Type: Full Time
Salary: NIH standard or higher
Years of Experience:
Less than 2
I am looking for a postdoc to work on double-strand break repair in yeast. More specifically, we are interested in the origin of mutations arising during gene editing and other types of DNA repair.
We have previously found that the mutation rate accompanying double-strand break repair is 1000 times higher than seen for spontaneous mutations in the same sequences. Moreover, the spectrum of mutations is very different, with many mutations reflecting the instability of the DNA repair polymerase complex, leading to increases in structural variants: deletions, tandem duplications, quasipalindrome mutations and interchromosomal template switches, even when the second template, on a different chromosome, is only 72% identical to the initial donor template, first described in (https://pubmed.ncbi.nlm.nih.gov/20595613/; https://pubmed.ncbi.nlm.nih.gov/25066232/). Most recently we have been studying mutations arising during Cas9-mediated gene editing. We have also devised specific assays for different types of structural variants and are interested in how alterations of the DNA repair machinery affect these types of mutations.
Applicants should send a CV and their thoughts about what they would like to work on to Jim Haber (firstname.lastname@example.org). Brandeis University, committed to diversity, equality and inclusion, is about 10 miles from the center of Boston/Cambridge, easily reached by public transportation.
Previous experience working in yeast is not necessary. We will train you in all the necessary techniques. But PhD-level experience in genetics and in molecular biology is required.