Research focused on understanding how herpes simplex virus (HSV) proteins and virus particles are transported in neuronal axons. The position will involve manipulating and producing plasmid DNAs, which will be transfected into neurons cells to produce HSV proteins that are transported into neuronal axons. Other work will involve studies of protein-protein interactions to understand which HSV proteins interact with kinesin motors that transport virus particles in axons. The position requires that the candidate have experience with molecular biology: characterizing proteins using polyacrylamide gels and western blots, manipulating plasmid DNAs, cell culture and transfection of cells. Laboratory experience with manipulating and expressing plasmids and with mammalian cell culture and transfection of cells is essential. Experience with handling viruses such as HSV or other viruses will also be valuable. Experience with culturing neurons will also be very helpful. Applicants must have a BSc, although those with MSc or PhD degrees and experience will be given priority.
Function/Duties of Position
Manipulating and producing plasmid DNAs, which will be transfected into neurons to characterize protein transport into axons.
Producing virus stocks and using these viruses to infect neurons
Passaging and caring for neuronal cell lines and imaging these cells using confocal microscopy in order to determine how viruses and viral proteins are transported into neuronal axons
Supervising an RA1 who will order supplies, wash dishes, do routine maintenance in the lab and perform experiments.
Preparing representations of data and working with the PI to write scientific papers and write grant applications.
Bachelor's in relevant field AND 1 years of relevant experience OR
Equivalent combination of training and experience
Laboratory experience with manipulating and expressing plasmids and with mammalian cell culture and transfection of cells is essential.
Experience with transfecting cells, propagating viruses and culturing neurons will also be very valuable.
Full time days, location RJH, standard microbiology laboratory conditions, exposures such as hazardous chemicals and biohazard waste. Will perform common laboratory protocols and tissue culture work.
All are welcome
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