• The Department of Genetic Medicine is seeking a Research Technologist to carry out the daily tasks of a growing Core facility laboratory. This position will set up sequencing reactions, gene expression and genotyping assays, distribute oligonucleotides and other products to customers, interact with and assist customers using self-service qPCR equipment. This position will interact with students, technicians and primary investigators as needed, including troubleshooting customer samples and experiments. This position must be able to provide exceptional customer service and maintain meticulous work standards.

Specific Duties & Responsibilities

• With minimal supervision, follow protocols to carry out sequencing reactions, gene expression assays, genotyping assays, pyrosequencing, digital PCR and other experiments as necessary.
• Set up sequencing reactions and run DNA Sequencer.
• Set up gene expression/genotyping assays and run associated equipment. Follow QA/QC protocols and all laboratory safety protocols.
• Responsible for daily maintenance of the equipment and proper use. Monitor laboratory supplies and order supplies when needed.
• Operate the pyrosequencing and digital PCR instruments independently. Assess data and release to customers.
• Contact, interact with, and assist customers as needed.
• Troubleshoot customer experiments and provide assistance as needed. Keep track of self-service genotyping use and maintain iLab billing records. Ensure that client requests are entered properly into database.
• Ensure that samples are properly labeled and match requests.
• Receive oligonucleotide delivers and orders and update database as necessary.
• Receive, log and track orders for delivery to our off-site locations. Deliver oligonucleotides and other products to customers as needed.
• Answer phones when needed.
• Willing to cross-train in human blood processing, cell culture and single cell genomic methods.
• Monitor database.
• Perform miscellaneous related duties as assigned.
• Cross-train to other GRCF centers as needed.

Machines/Equipment Used

• Experience with the following equipment helpful but will train as necessary.
• Centrifuges, electrophoresis equipment, 3730XL DNA Analyzers, Real-Time PCR equipment (including the QuantStudio qPCR instruments), Digital PCR, PCR thermocycler, robotic and manual pipettors, common lab equipment (hotplate/stirrers, balances, spectrophotometer, etc.), Macintosh and IBM computers; 95% time.

Specific Physical Requirements

• Sitting in a normal seated position for extended periods of time. Reaching by extending hand(s) or arm(s) in any direction.
• Finger dexterity to manipulate objects with fingers rather than with whole hand or arm, for example, using a keyboard.
• Communication skills using the spoken word. Ability to see and hear within normal parameters. Ability to move about workspace.
• Lift, carry, move supplies, computers, etc.

• Bachelor's Degree in Biology, Chemistry or related field.

• Experience in a Core facility setting.
• Some related post-undergraduate work experience preferred.

Classified Title: Research Technologist 
Role/Level/Range: ACRO40/E/02/CC  
Starting Salary Range: $15.70 - $26.25 HRLY ($43,680 targeted; Commensurate with experience) 
Employee group: Full Time 
Schedule: 8:30-5:00pm (some flex if needed) 
Exempt Status: Non-Exempt 
Location: School of Medicine Campus 
Department name: ???????SOM Genetic Resources Core Facility GRCF  
Personnel area: School of Medicine 

About Johns Hopkins University

Johns Hopkins University remains committed to its founding principle, that education for all students should be grounded in exploration and discovery. Hopkins students are challenged not just to learn but also to advance learning itself. Critical thinking, problem solving, creativity, and entrepreneurship are all encouraged and nourished in this unique educational environment. After more than 130 years, Johns Hopkins remains a world leader in both teaching and research. Faculty members and their research colleagues at the university's Applied Physics Laboratory have each year since 1979 won Johns Hopkins more federal research and development funding than any other university. The university has nine academic divisions and campuses throughout the Baltimore-Washington area. The Krieger School of Arts and Sciences, the Whiting School of Engineering, the School of Education and the Carey Business School are based at the Homewood campus in northern Baltimore. The schools of Medicine, Public Health, and Nursing share a campus in east Baltimore with The Johns Hopkins Hospital. The Peabody Institute, a leading professional school of music, is located on Mount Vernon Place in downtown Bal...timore. The Paul H. Nitze School of Advanced International Studies is located in Washington's Dupont Circle area.

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